Private channel session 1335
32 messages over 2h 47m, 2021-05-06 – 2021-05-06.
A “conversation” here is an activity session — a run of messages with under 60 minutes of silence inside it. The channel had no native conversation boundaries.
-
Next to my Yunnan elephant [shared file(s): IMG_1633.JPG]
-
Looks great Eddie!
-
It's great! Thanks so much.
-
Haha. Now, put that CCP award next to it and we have a perfect setup.
-
More from Daszak. I'm not sure if I'm comforted by the 15,000 bat samples in the freezer: "I keep making the point that the US taxpayer paid for us to collect 15,000+ bat samples that are now in freezers in Wuhan, and because ofthis fake cold-war we can't officially control the work on them. Zhengli's doing the right thing - we're acting just likewe were when the NIH grant was up and running, but if they decided not to answer my emails or wechats, we've gotno leverage. Dumb politics."
-
15,000 bat samples in the freezer.... Awesome. :wink:
-
Also - just found this oldie but goodie. I believe the likes of eDim and Alina have forced me into a more extreme position.... Which I'll continue to defend! :punch:https://andersenlab.slack.com/archives/GTGL6LY5C/p1591918962047400
-
I remember that well! By the way, there are no prior publications of Shi using FCSs are there? So, if this were from the lab it would their first use on the mystery virus they've told no one about.
-
I don't think so - that's kinda what I'm trying to figure out...
-
I'm getting a TON of shit because of Wade's article, so I need to look a little closer at all of this - just for my ownsake. I'm not going to respond to Wade, but I have colleagues at UCSD telling me that _they_ have been receiving Wade's article from _their_ colleagues. Some twisted shit.
-
Yes, ignore Wade. I wonder if they've (Shi) ever even published a natural bat CoV with a FCS?
-
Probably the closest example: https://pubmed.ncbi.nlm.nih.gov/26063432/
-
No engineering in this one, but also about cleavage. https://pubmed.ncbi.nlm.nih.gov/31801868/
-
I'm surprised this virus didn't emerge in Raleigh.
-
Yeah, me too.
-
Quickly on Wade: pretty amazing that he accuses us of bad science when he ignores ALL the counter data.
-
Yup. I expect RelBloom will do the same.
-
When it comes to Baric fiddling with viruses, this, of course, is the mother of all papers... https://www.nature.com/articles/s41591-020-0924-2
-
Well, this https://www.nature.com/articles/nm.3985
-
And it shows the key point: you use a standard backbone when you do this GOF work. And surely Shi would have done the same as she collaborated with Baric? And surely she would have asked Baric for advice but there's nothing in his emails?
-
Yup. Exactly.
-
First experiment to stick the SC2 RBD in a mouse adapted strain.
-
Yeah - very likely, but depends on the purpose of the experiment.
-
And just for reference - here are those beautiful feline CoVs with an identical FCS motif. It's out there... [shared file(s): Screen Shot 2021-05-06 at 9.07.11 PM.png]
-
Yes, I've used this one in talks
-
Sometimes I fucking love science. One of Wade's biggest arguments is that (and this is an oldie but goodie...) the FCS is strange because it has those two CGG codons coding the arginines. Now, take a look at the Feline CoVs -first R is coded by CGG too. Totally sinks that entire argument. [shared file(s): Screen Shot 2021-05-06 at 9.19.12PM.png]
-
Perfect!!
-
Don't you love nature!!
-
Looking at the CGG codon itself and how often it's used: Common cold CoVs: 4% SARS: 5% SARS2: 3% SARSrCoVs: 2% Totally sinks the argument.
-
Hilarious!
-
And one more - if CGGCGG had been 'strange' in SARS-CoV-2, we would see plenty of synonymous mutations (R has six codons). What do we see if we analyze the ~1.5 million genomes? CGG is found in 99.84% of all sequences at that site. Not exactly unstable. I can go to bed now. I'll spend some time on the rest of his arguments tomorrow. Just because it's fun...
-
Great job! Get some rest.