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Slack / Private Message Drop, pp.757-758 [SLACK_000971] · slack_pm:msg:07934

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First experiment to stick the SC2 RBD in a mouse adapted strain.

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2021-05-06 20:42 Kristian G. Andersen open

When it comes to Baric fiddling with viruses, this, of course, is the mother of all papers... https://www.nature.com/articles/s41591-020-0924-2
2021-05-06 20:42 Kristian G. Andersen open

Well, this https://www.nature.com/articles/nm.3985
2021-05-06 20:52 Edward C. Holmes open

And it shows the key point: you use a standard backbone when you do this GOF work. And surely Shi would have done the same as she collaborated with Baric? And surely she would have asked Baric for advice but there's nothing in his emails?
2021-05-06 20:55 Kristian G. Andersen open

Yup. Exactly.
2021-05-06 20:55 Edward C. Holmes

First experiment to stick the SC2 RBD in a mouse adapted strain.
2021-05-06 20:57 Kristian G. Andersen open

Yeah - very likely, but depends on the purpose of the experiment.
2021-05-06 21:08 Kristian G. Andersen open

And just for reference - here are those beautiful feline CoVs with an identical FCS motif. It's out there... [shared file(s): Screen Shot 2021-05-06 at 9.07.11 PM.png]
2021-05-06 21:21 Edward C. Holmes open

Yes, I've used this one in talks
2021-05-06 21:21 Kristian G. Andersen open

Sometimes I fucking love science. One of Wade's biggest arguments is that (and this is an oldie but goodie...) the FCS is strange because it has those two CGG codons coding the arginines. Now, take a look at the Feline CoVs -first R is coded by CGG too. Totally sinks that entire argument. [shared file(s): Screen Shot 2021-05-06 at 9.19.12PM.png]