Slack / Private Message Drop — page 57

a-subunit (ENaC-a) how looks in other species? Would be interesting to know a is? [2020-06-06 02:29:09] [Robert Garry] SARS-CoV-2 strategically mimics proteolytic activation of human ENaC [2020-06-06 02:29:12] [Robert Garry] human epithelial sodium channel
-subunit (ENaC-
) [2020-06-06 02:38:33] [Robert Garry] Sorry - messed up the links above - the elife paper does a considerable amt of arm waving over an 8 amino acid homology -the furin site - but "interesting" that the furin site in both SARS-CoV-2 and ENaC-
has predicted O-linked glycans PLUS ENaC-
has another proline, serine, threonine rich stretch - a likely mucin domain - OC43 and HKU1 Spike also have this. [2020-06-06 02:51:28] [Robert Garry] "RTT plot for Ebola is super interesting" Agree - how do the RBDs align on the different branches? [2020-06-06 03:10:21] [Eddie Holmes] Got it. I don't suppose we know much about how ENaC-
how looks in other species? Would be interesting to know what species OC43 and HKU1 originated in. [2020-06-06 03:20:32] [Eddie Holmes] Going through our scenarios, if true would the ENaC-
+ furin link argue for undetected circulation in humans? Possibly to get in animal passage? Could it explain why the furin site is sometimes lost in cell culture? It is clearly doing something. Bill's theory explains how it was created, but it would also be nice to understand how it is maintained. [2020-06-06 03:28:10] [Andrew Rambaut] Sorry - I am being dumb here. Can you layout exact what the proposed connection with ENaC-
is? [2020-06-06 03:29:05] [Eddie Holmes] shared file(s): elife-58603-v1.pdf [2020-06-06 03:29:13] [Eddie Holmes] Figure 1 [2020-06-06 03:30:21] [Andrew Rambaut] But not suggesting this was the source of the insertion recombination? [2020-06-06 03:30:59] [Andrew Rambaut] Just why it might be doing in humans. [2020-06-06 03:31:33] [Eddie Holmes] No. But it explains why it is beneficial. A bit of undetected circulation in humans would solve a lot of problems. [2020-06-06 03:37:32]