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Slack / Private Message Drop, p.57 [SLACK_000271] · slack_pm:msg:00569

Page text: p.57 · original PDF

Date
2020-06-06 02:51
Type
chat message · slack
recipient
Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker
Robert F. Garry
Topics
Furin cleavage site and molecular features

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"RTT plot for Ebola is super interesting" Agree - how do the RBDs align on the different branches?

Extracted statements

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StatementGradeAttributionStance
"RTT plot for Ebola is super interesting" Agree - how do the RBDs align on the different branches? quoted / not their view quoted_external inside quotation marks questions

In context

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  1. 2020-06-06 02:28 Robert F. Garry open
    https://elifesciences.org/articles/58603 a-subunit (ENaC-a) how looks in other species? Would be interesting to know a is?
  2. 2020-06-06 02:29 Robert F. Garry open
    SARS-CoV-2 strategically mimics proteolytic activation of human ENaC
  3. 2020-06-06 02:29 Robert F. Garry open
    human epithelial sodium channel -subunit (ENaC-)
  4. 2020-06-06 02:38 Robert F. Garry open
    Sorry - messed up the links above - the elife paper does a considerable amt of arm waving over an 8 amino acid homology -the furin site - but "interesting" that the furin site in both SARS-CoV-2 and ENaC- has predicted O-linked glycans PLUS ENaC- has another proline, serine, threonine rich stretch - a likely mucin domain - OC43 and HKU1 Spike also have this.
  5. 2020-06-06 02:51 Robert F. Garry
    "RTT plot for Ebola is super interesting" Agree - how do the RBDs align on the different branches?
  6. 2020-06-06 03:10 Edward C. Holmes open
    Got it. I don't suppose we know much about how ENaC- how looks in other species? Would be interesting to know what species OC43 and HKU1 originated in.
  7. 2020-06-06 03:20 Edward C. Holmes open
    Going through our scenarios, if true would the ENaC- + furin link argue for undetected circulation in humans? Possibly to get in animal passage? Could it explain why the furin site is sometimes lost in cell culture? It is clearly doing something. Bill's theory explains how it was created, but it would also be nice to understand how it ismaintained.
  8. 2020-06-06 03:28 Andrew Rambaut open
    Sorry - I am being dumb here. Can you layout exact what the proposed connection with ENaC- is?
  9. 2020-06-06 03:29 Edward C. Holmes open
    shared file(s): elife-58603-v1.pdf

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