Slack / Private Message Drop — page 58

[Andrew Rambaut] I see it now. Interesting. [2020-06-06 03:38:30] [Andrew Rambaut] But then we have the source of the nucleotides to think about? [2020-06-06 03:42:19] [Andrew Rambaut] Just when I think I have this thing straight in my mind. [2020-06-06 03:53:17] [Andrew Rambaut] [thread - ID: 2020-06-05 12:35:54] @Robert Garry Do you have the nucleotide sequence for this? Could it be the actual source of the insertion? [2020-06-06 04:21:16] [Andrew Rambaut] This is interesting: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5312086/ [2020-06-06 04:21:45] [Andrew Rambaut] shared file(s): image.png [2020-06-06 04:24:09] [Andrew Rambaut] Suggests a progressive accumulation of the polybasic site in passaging. [2020-06-06 05:28:49] [Robert Garry] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7106199/ [2020-06-06 05:29:11] [Robert Garry] "BCoV is closely related to the Human coronavirus OC43 (HCoV-OC43), that was first isolated in 1967 (McIntosh et al., 1967). More precisely, BCoV and HCoV-OC43 share a global nucleotide identity of 96%. The genetic comparison of BCoV and HCoV-OC43 reveals that HCoV-OC43 results from a zoonotic transmission from bovine to human." [2020-06-06 07:28:13] [Robert Garry] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7112090/ [2020-06-06 07:38:51] [Robert Garry] Very strong review on the topic from Drosten. Doesn't mean there isn't room for a much deeper dive using current genomics tools. [2020-06-06 09:21:58] [Kristian Andersen] The eLife and flu 'polymerase slippage' papers are very interesting indeed. As to how beneficial the furin site is in humans, I thought this one was reasonably interesting (although I don't believe we have seen this in our sequences - we'll dig deeper):