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Slack / Private Message Drop, p.57 [SLACK_000271] · slack_pm:msg:00571

Date: 2020-06-06 03:20
Type: chat message · slack
recipient: Kristian G. Andersen, Robert F. Garry, Andrew Rambaut
speaker: Edward C. Holmes
Topics: Furin cleavage site and molecular features

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Going through our scenarios, if true would the ENaC- + furin link argue for undetected circulation in humans? Possibly to get in animal passage? Could it explain why the furin site is sometimes lost in cell culture? It is clearly doing something. Bill's theory explains how it was created, but it would also be nice to understand how it ismaintained.

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Statement	Grade	Attribution	Stance
Could it explain why the furin site is sometimes lost in cell culture?	needs context	speaker_own	questions

Statement

Grade

Attribution

Stance

Could it explain why the furin site is sometimes lost in cell culture?

needs context

speaker_own

questions

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2020-06-06 02:29 Robert F. Garry open

human epithelial sodium channel -subunit (ENaC-)
2020-06-06 02:38 Robert F. Garry open

Sorry - messed up the links above - the elife paper does a considerable amt of arm waving over an 8 amino acid homology -the furin site - but "interesting" that the furin site in both SARS-CoV-2 and ENaC- has predicted O-linked glycans PLUS ENaC- has another proline, serine, threonine rich stretch - a likely mucin domain - OC43 and HKU1 Spike also have this.
2020-06-06 02:51 Robert F. Garry open

"RTT plot for Ebola is super interesting" Agree - how do the RBDs align on the different branches?
2020-06-06 03:10 Edward C. Holmes open

Got it. I don't suppose we know much about how ENaC- how looks in other species? Would be interesting to know what species OC43 and HKU1 originated in.
2020-06-06 03:20 Edward C. Holmes

Going through our scenarios, if true would the ENaC- + furin link argue for undetected circulation in humans? Possibly to get in animal passage? Could it explain why the furin site is sometimes lost in cell culture? It is clearly doing something. Bill's theory explains how it was created, but it would also be nice to understand how it ismaintained.
2020-06-06 03:28 Andrew Rambaut open

Sorry - I am being dumb here. Can you layout exact what the proposed connection with ENaC- is?
2020-06-06 03:29 Edward C. Holmes open

shared file(s): elife-58603-v1.pdf
2020-06-06 03:29 Edward C. Holmes open

Figure 1
2020-06-06 03:30 Andrew Rambaut open

But not suggesting this was the source of the insertion recombination?

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