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Slack / Private Message Drop, p.57 [SLACK_000271] · slack_pm:msg:00568

Date: 2020-06-06 02:38
Type: chat message · slack
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry
Topics: Furin cleavage site and molecular features

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Sorry - messed up the links above - the elife paper does a considerable amt of arm waving over an 8 amino acid homology -the furin site - but "interesting" that the furin site in both SARS-CoV-2 and ENaC- has predicted O-linked glycans PLUS ENaC- has another proline, serine, threonine rich stretch - a likely mucin domain - OC43 and HKU1 Spike also have this.

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Statement	Grade	Attribution	Stance
Sorry - messed up the links above - the elife paper does a considerable amt of arm waving over an 8 amino acid homology -the furin site - but "interesting" that the furin site in both SARS-CoV-2 and ENaC- has predicted O-linked glycans PLUS ENaC- has another proline, serine, threonine rich stretch - a likely mucin domain - OC43 and HKU1 Spike also have this.	quoted / not their view	quoted_external sentence carries quotation marks	asserts

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quoted / not their view

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2020-06-06 02:28 Robert F. Garry open

https://elifesciences.org/articles/58603 a-subunit (ENaC-a) how looks in other species? Would be interesting to know a is?
2020-06-06 02:29 Robert F. Garry open

SARS-CoV-2 strategically mimics proteolytic activation of human ENaC
2020-06-06 02:29 Robert F. Garry open

human epithelial sodium channel -subunit (ENaC-)
2020-06-06 02:38 Robert F. Garry

Sorry - messed up the links above - the elife paper does a considerable amt of arm waving over an 8 amino acid homology -the furin site - but "interesting" that the furin site in both SARS-CoV-2 and ENaC- has predicted O-linked glycans PLUS ENaC- has another proline, serine, threonine rich stretch - a likely mucin domain - OC43 and HKU1 Spike also have this.
2020-06-06 02:51 Robert F. Garry open

"RTT plot for Ebola is super interesting" Agree - how do the RBDs align on the different branches?
2020-06-06 03:10 Edward C. Holmes open

Got it. I don't suppose we know much about how ENaC- how looks in other species? Would be interesting to know what species OC43 and HKU1 originated in.
2020-06-06 03:20 Edward C. Holmes open

Going through our scenarios, if true would the ENaC- + furin link argue for undetected circulation in humans? Possibly to get in animal passage? Could it explain why the furin site is sometimes lost in cell culture? It is clearly doing something. Bill's theory explains how it was created, but it would also be nice to understand how it ismaintained.
2020-06-06 03:28 Andrew Rambaut open

Sorry - I am being dumb here. Can you layout exact what the proposed connection with ENaC- is?

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