Slack / Private Message Drop — page 527

ORF1b P314L? Interesting - pretty much defines Doug... [2021-02-19 13:31:39] [Robert Garry] Yeah - DouG literally opened up the stem but the nsp12 mutation may be affecting polymerase fidelity (surprising if it didn't). Could be why @Eddie Holmes reports the volutility in trying to grow postDouG variants in cell culture. [2021-02-19 13:32:33] [Robert Garry] P323L I think. [2021-02-19 13:34:19] [Kristian Andersen] Here's Nextstrain - 323 is listed as only "K". [shared file(s): Screen Shot 2021-02-19 at 1.33.44 PM.png] [2021-02-19 13:34:41] [Kristian Andersen] https://nextstrain.org/ncov/global?c=gt-ORF1b_314 [2021-02-19 14:17:22] [Robert Garry] Yeah -that it... It's DouG's world but his naughty polymerase girlfriend PearL may be causing a lot of disruption on her own. [2021-02-19 14:26:29] [Kristian Andersen] You know - none of these spike mutations may make SARS-CoV-2 more transmissible... [2021-02-19 14:30:01] [Robert Garry] You could be right - Thinking back and Bob C and Tom Geisbert will know better, but I think that all of the mutations that make LASV replicate in guinea pigs are in polymerase. [2021-02-19 14:34:17] [Kristian Andersen] I believe in flu, the polymerase is key too. [2021-02-19 14:38:45] [Robert Garry] Exactly! [2021-02-19 14:39:34] [Robert Garry] Although occasionally a little FCS can get involved in the fun. [2021-02-19 14:45:42] [Andrew Rambaut] I heard from a confidential source that in macaque infections P323L (Phil) came up in 10/10 monkeys. [2021-02-19 14:46:48] [Andrew Rambaut] And yes Phil is strongly linked with Doug but came up first (in January). [2021-02-19 14:47:34] [Andrew Rambaut]