Chat message

Slack / Private Message Drop, p.527 [SLACK_000741] · slack_pm:msg:05488

Date: 2021-02-19 13:31
Type: chat message · slack
mentioned: Edward C. Holmes
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry

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Yeah - DouG literally opened up the stem but the nsp12 mutation may be affecting polymerase fidelity (surprising if itdidn't). Could be why @Eddie Holmes reports the volutility in trying to grow postDouG variants in cell culture.

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2021-02-19 12:47 Robert F. Garry open

Looks like it showed up early around about the time of the first DouG sightings.
2021-02-19 13:12 Kristian G. Andersen open

What's the coordinates of that mutation in ORF1a space Bob?
2021-02-19 13:24 Robert F. Garry open

I think it's C14408T P4715L.
2021-02-19 13:27 Kristian G. Andersen open

ORF1b P314L? Interesting - pretty much defines Doug...
2021-02-19 13:31 Robert F. Garry

Yeah - DouG literally opened up the stem but the nsp12 mutation may be affecting polymerase fidelity (surprising if itdidn't). Could be why @Eddie Holmes reports the volutility in trying to grow postDouG variants in cell culture.
2021-02-19 13:32 Robert F. Garry open

P323L I think.
2021-02-19 13:34 Kristian G. Andersen open

Here's Nextstrain - 323 is listed as only "K". [shared file(s): Screen Shot 2021-02-19 at 1.33.44 PM.png]
2021-02-19 13:34 Kristian G. Andersen open

https://nextstrain.org/ncov/global?c=gt-ORF1b_314
2021-02-19 14:17 Robert F. Garry open

Yeah -that it... It's DouG's world but his naughty polymerase girlfriend PearL may be causing a lot of disruption onher own.

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