Slack / Private Message Drop — page 271

Here's the reference for the FCS control: https://pubmed.ncbi.nlm.nih.gov/28924042/ [2020-11-21 07:57:21] [Robert Garry] Yes - very pertinent which means I need to add this reference and you as a co-author -you already pointed me to the feline CoVs OK? Also OK to add Andrew and Eddie in acknowledgents for helpful discussions and encouragement? Alternatively I think Andrew and Eddie rise to authorship as well - putting all the PO1 authors on minus Ian but plus Prof Bill in A Virological would certainly garner some attention and stir up the Chan, Yan , Relman and Billy krewe. [2020-11-21 08:03:46] [Kristian Andersen] I think this is a clear Bob Garry investigation, so no need to add me - but yeah, add us all in acknowledgments :wink: [2020-11-21 08:04:44] [Kristian Andersen] [unless you're scared to face the trolls on your own... :wink: ] [2020-11-21 08:04:51] [Andrew Rambaut] It would be interesting to test an SC2 with a FCS deletion in ferrets [2020-11-21 08:06:35] [Kristian Andersen] Agreed - Bob and I have discussed these types of experiments previously. Would also be important to do the same in e.g., human lung cells. Don't know if there's an isolate without the FCS though? [2020-11-21 08:12:13] [Robert Garry] The deletion is nonpathogenic in hamsters, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7457603/ but yes ferret a better model. Tulane primate center and other doing monkeys but I don't know latest results. [2020-11-21 08:13:47] [Kristian Andersen] Yeah, ferrets better - and question is - will it pick it up if passaged in ferrets? I bet this whole thing could easily relate to farming of mustelids. [2020-11-21 09:42:51] [Robert Garry] This was Yoshi's H5 flu experiment: "After 24 consecutive passages by air sac inoculation, followed by five passages in chicken brain, the avirulent virus became highly pathogenic in chickens, producing a 100% mortality rate. Sequence analysis at the hemmaglutinin cleavage site of the original isolate revealed a typical avirulence type of sequence, R-E-T-R, which progressed incrementally to a typical virulence type of sequence, R-R-K-K-R, during repeated passages in chickens." That's a lot of ferrets for 29 passaages which aren't cheap, but worth the effort. [2020-11-21 09:48:26] [Kristian Andersen] Denmark has a lot of cheap mink up for sale... [2020-11-21 09:56:14] [Robert Garry] Sad but true - you should suggest they put some to a better use than yet another coat for the likes of Melania, Ivanka et al. [2020-11-21 09:59:20] [Robert Garry] Of course with Donny Jr the latest Trump to get infected that crime family doing it's best at "serial passage."