A single line often inverts meaning once you see what it
answers, so neighbouring messages are always shown.
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Andrew, yes, thanks for sharing - great points in there. "Did the acquisition of the ACE2 RBD make the virus more species-promiscuous and thus facilitated emergence into farmed carnivores like raccoon-dogs/pangolins? Highdensity transmission in carnivores may have evolved the FCS - i.e., like chickens & high-path AIV."I actually think that's due to the FCS - it's been shown before that FCS increases the hostrange of other coronaviruses, including MERS-like - one reference: https://pubmed.ncbi.nlm.nih.gov/31801868/. Key sentence here: "Together, these results indicate that proteolytic cleavage of the spike, not receptor binding, is the primary infection barrier for these two group 2c CoVs".
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some of the serpentoviruses and nonCoV nidos have interesting mucin-like domains
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The O-linked glycans very likely regulates the FCS site - I don't think it's a mucin-like domain
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agree - thou with some of the other Nido spikes there are more than a dozen o-linked sites so mucin-like
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2020-11-21 07:48
Kristian G. Andersen
Here's the reference for the FCS control: https://pubmed.ncbi.nlm.nih.gov/28924042/
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Yes - very pertinent which means I need to add this reference and you as a co-author -you already pointed me to the feline CoVs OK? Also OK to add Andrew and Eddie in acknowledgents for helpful discussions and encouragement?Alternatively I think Andrew and Eddie rise to authorship as well - putting all the PO1 authors on minus Ian but plus Prof Bill in A Virological would certainly garner some attention and stir up the Chan, Yan , Relman and Billy krewe.
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I think this is a clear Bob Garry investigation, so no need to add me - but yeah, add us all in acknowledgments :wink:
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[unless you're scared to face the trolls on your own... :wink: ]
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It would be interesting to test an SC2 with a FCS deletion in ferrets