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Slack / Private Message Drop, p.70 [SLACK_000284] · slack_pm:msg:00712

Page text: p.70 · original PDF

Date: 2020-06-11 15:16
Type: chat message · slack
recipient: Robert F. Garry, Edward C. Holmes, Andrew Rambaut
speaker: Kristian G. Andersen

Recipients on this medium are inferred from channel membership, not per-message addressing.

(BUT, the inserted site _is not_ the same as in the paper - that would have been a dead giveaway)

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2020-06-11 15:13 Kristian G. Andersen open

People have put furin sites into CoVs previously - here's one example from SARS: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7111780/
2020-06-11 15:13 Kristian G. Andersen open

There's a paper where they put it into a bat virus and shows it has much broader host range - I believe from Baric's group. I'll find it.
2020-06-11 15:15 Edward C. Holmes open

At S1/S2?
2020-06-11 15:15 Kristian G. Andersen open

Yup - the paper above is _exactly_ where it was inserted in SARS2 - that's the part I missed previously (I have been aware of these papers all the time).
2020-06-11 15:16 Kristian G. Andersen

(BUT, the inserted site _is not_ the same as in the paper - that would have been a dead giveaway)
2020-06-11 15:16 Edward C. Holmes open

We're going round in circles...that's the Follis paper...the first paper we discussed and I sent you in early Feb.
2020-06-11 15:18 Edward C. Holmes open

My take is that furin cleavage sites are *everywhere* in natural viruses e.g. MERS. I'm revising a paper on avianavulaviruses and they have one. To me, there is a strong natural prior.
2020-06-11 15:18 Kristian G. Andersen open

Yes - I have been aware of the Follis paper all along - I was _not_ aware that their insertion was exactly where it is inSARS2
2020-06-11 15:20 Edward C. Holmes open

Also, the Zhou et al. paper never call it a furin cleavage site insertion and I think the alignment in that paper is the optimal one. If so, it is not a simple 'shift' but rather a complex set of indel events.

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