A single line often inverts meaning once you see what it
answers, so neighbouring messages are always shown.
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Yup - the paper above is _exactly_ where it was inserted in SARS2 - that's the part I missed previously (I have been aware of these papers all the time).
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(BUT, the inserted site _is not_ the same as in the paper - that would have been a dead giveaway)
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We're going round in circles...that's the Follis paper...the first paper we discussed and I sent you in early Feb.
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My take is that furin cleavage sites are *everywhere* in natural viruses e.g. MERS. I'm revising a paper on avianavulaviruses and they have one. To me, there is a strong natural prior.
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2020-06-11 15:18
Kristian G. Andersen
Yes - I have been aware of the Follis paper all along - I was _not_ aware that their insertion was exactly where it is inSARS2
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Also, the Zhou et al. paper never call it a furin cleavage site insertion and I think the alignment in that paper is the optimal one. If so, it is not a simple 'shift' but rather a complex set of indel events.
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Here's the paper from Baric - not the insertion, but showing that cleavage broadens host range (https://pubmed.ncbi.nlm.nih.gov/31801868/) - which is what furin does. This is also not a new paper - I have been aware of this all along as well, so not a smoking gun, but there are several concerns here related to tissue culture
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I don't think the Zhou et al. paper shows that this is multiple indels - to me it just looks like a region not aligning very well?
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Let me spend some time on a table with things that bothers me - because there are several.