Slack / Private Message Drop — page 184

[2020-09-18 16:18:31] [Kristian Andersen] Yeah, RmYN02 - the more complete of the two genomes. Definitely another genome in there [2020-09-18 16:20:30] [Kristian Andersen] It's another bat CoV closely related (but not identical) to others previously sequenced. Every yellow bar is an intrahost variant >10% (SNPs are all linked as far as I can tell, so I think it's just one additional genome) [shared file(s): Screen Shot 2020-09-18 at 16.19.28.png] [2020-09-18 16:25:42] [Eddie Holmes] Yes, I agree. I'll check back what we did - if anything - about this. It would be really interesting to see a phylogeny with that, RmYN02 and RmYN01 included. [2020-09-18 16:37:13] [Kristian Andersen] I'm just going to pull that minority genome out - I'll keep you posted... Yeah, this is very interesting [2020-09-18 16:39:04] [Eddie Holmes] Great! [2020-09-18 17:14:52] [Kristian Andersen] Here's what I have assembled so far - all look great. Only need to assemble one of the remaining pangolin CoVs, which has a huge dataset. RBD in green. Conclusion? Nothing to see here.... as expected. [shared file(s): MP789.jpg, P1E.jpg, P2S.jpg, P2V.jpg, P3B.jpg, P4L.jpg, P5E.jpg, P5L.jpg, RaTG13.jpg, RmYN02.jpg] [2020-09-18 17:40:16] [Eddie Holmes] Great work! So annoying that these people are accusing of fabrication when they are honest scientists working hard. Makes by blood boil. [2020-09-18 18:03:12] [Kristian Andersen] Here's the minor genome - probably not a complete genome, but definitely present. [shared file(s): minor_variant_genome.fa] [2020-09-18 18:03:31] [Kristian Andersen] Alignment + tree [shared file(s): Alignment with minor RmYN02 RAxML Tree.nex, Alignment with minor RmYN02.nex] [2020-09-18 18:03:54] [Eddie Holmes] Cool! Quick tree? [2020-09-18 18:05:24] [Kristian Andersen] And some stats. This is all some fast hacked together stuff, so the boys should take a closer look.