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Slack / Private Message Drop, p.1029 [SLACK_001243] · slack_pm:msg:10753

Date: 2021-11-25 08:22
Type: chat message · slack
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry

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I can model it - not possible to get a structure. It has probably 5 TM domains, which you expect of a membrane remodeler. The TMpred prediction is underlined and I bolded the "by-eye" most likely TMs. There are three internal domains that are in blue and italicized. There is a large external domain that has several cysteines -dicysteines - it'san uneven number so maybe a metal binding spot. The third external domain has a lot of aromatic amino acids - sodoes the putative fifth TM domain so that's probably the business part of the membrane remodeling. The T492Imutation is shown - it's at the end of the molecule - inside - 4 cysteines that domain could be regulatory.

In context

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2021-11-25 07:07 Kristian G. Andersen open

Bob - do we know anything about NSP4? B.1.1.529 is sharing T492I with an extremely rapidly growing lineage in Chile (although I suspect that must almost certainly be doing to epi factors, not that particular mutation, but :man-shrugging:): https://outbreak.info/situation-reports?pango&muts=ORf1b%3AT2163I&selected=CHL &loc=USA&loc=USA_US-CA&loc=CHL&overlay=false
2021-11-25 07:32 Andrew Rambaut open

Tulio starts at 26 mins
2021-11-25 08:13 Robert F. Garry open

It's involved in remodeling the internal membranes - that where the transcription/replication takes place. [shared file(s): image.png]
2021-11-25 08:16 Andrew Rambaut open

This evening I think we may be back to square one with respect to vaccination and prior immunity. New pandemic anyone?
2021-11-25 08:22 Robert F. Garry

I can model it - not possible to get a structure. It has probably 5 TM domains, which you expect of a membrane remodeler. The TMpred prediction is underlined and I bolded the "by-eye" most likely TMs. There are three internal domains that are in blue and italicized. There is a large external domain that has several cysteines -dicysteines - it'san uneven number so maybe a metal binding spot. The third external domain has a lot of aromatic amino acids - sodoes the putative fifth TM domain so that's probably the business part of the membrane remodeling. The T492Imutation is shown - it's at the end of the molecule - inside - 4 cysteines that domain could be regulatory.

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