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Slack / Private Message Drop, p.882 [SLACK_001096] · slack_pm:msg:09146

Page text: p.882 · original PDF

Date
2021-05-27 13:39
Type
chat message · slack
recipient
Kristian G. Andersen, Robert F. Garry, Edward C. Holmes
speaker
Andrew Rambaut

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Could do a COI amplification - i.e., species barcoding?

In context

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  1. 2021-05-27 13:06 Andrew Rambaut open
    Just discussing that. My personal hunch has been that B117 is adapted to strong NPI by being more transmissible early but for shorter (causing more damage and thus severity). Make the most of limited number of contacts. 617.2may have a longer pre-symptom period making it better in a more free-for-all environment (i.e., unfortunate timing asUK restrictions have lifted).
  2. 2021-05-27 13:10 Kristian G. Andersen open
    Yeah, that's an interesting theory - although it seems like 117 may actually also infect for longer? Did Oli ever do anyof the modeling analyses to try and flesh these differences out a little more?
  3. 2021-05-27 13:34 Edward C. Holmes open
    I wonder if they have enough material to shotgun sequence the environmental samples from the market? Might tell them want animals were being sold my looking at the expressed host genes (e.g. COII). Doesn't even need to be the virus positive samples although they would be better.
  4. 2021-05-27 13:38 Kristian G. Andersen open
    Well, fuck me - so obvious and honestly I had not thought about it. Yes, they should and yes that should be totallydoable.
  5. 2021-05-27 13:39 Andrew Rambaut
    Could do a COI amplification - i.e., species barcoding?
  6. 2021-05-27 13:47 Edward C. Holmes open
    I'll see if I can get George and Weifeng to do it
  7. 2021-05-27 13:50 Kristian G. Andersen open
    Yeah, they should really get on it - metagenomic sequencing should be sufficient. It'll be messy, but not a lot of data needed to do meaningful IDs
  8. 2021-05-27 13:52 Andrew Rambaut open
    You sure you would be able to do species ID? Pretty unlikely to bring up anything with species level specificity
  9. 2021-05-27 13:52 Andrew Rambaut open
    COI would be much more sensitive and there is the BOLD database

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