Chat message

Slack / Private Message Drop, p.1014 [SLACK_001228] · slack_pm:msg:10587

Date: 2021-10-03 08:23
Type: chat message · slack
mentioned: Robert F. Garry
recipient: Kristian G. Andersen, Robert F. Garry, Edward C. Holmes
speaker: Andrew Rambaut
Topics: Furin cleavage site and molecular features

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@Robert Garry The 'second round' takes place in the ER so might be more likely to have lots of other templates toswitch too, I guess. But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.

Extracted statements

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Statement	Grade	Attribution	Stance
But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.	quoted / not their view	hypothetical conditional framing	asserts

Statement

Grade

Attribution

Stance

But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.

quoted / not their view

hypothetical conditional framing

asserts

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2021-10-03 07:49 Robert F. Garry open

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908549/
2021-10-03 07:54 Robert F. Garry open

The old schoolers call the first round "replication" (generates the replicative intermediates). The second round they call "transcription"- generates the mRNAs.
2021-10-03 08:09 Andrew Rambaut open

Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs
2021-10-03 08:09 Andrew Rambaut open

Also I have always considered transcription to be DNA->RNA?
2021-10-03 08:23 Andrew Rambaut

@Robert Garry The 'second round' takes place in the ER so might be more likely to have lots of other templates toswitch too, I guess. But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.
2021-10-03 09:04 Robert F. Garry open

@Andrew Rambaut Agree - I don't like the terminology either - usually just say + to - or - to +. and for a positive virusof course as you say - to + can also be creating genomes so "replication" in my mind.
2021-10-03 09:05 Robert F. Garry open

Yes indeed - lots of the accessory proteins are just so the replicative intermediates can associate with membrane.

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