A single line often inverts meaning once you see what it
answers, so neighbouring messages are always shown.
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If the template is a related pos RNA virus rather than a mRNA in the same cell then you will have a lot of negativestrands. so the question of when the jump happens pos to neg or neg to pos is not an issue.
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Finally, Mike Worobey pinged me with a different but related question. He wants to do a virological post (and askedme - old school- to help). His hypothesis is that if there is a co-infection with BANAL-20-52 (NSRS at S1/S2) and oneof the RmYN02 like viruses such as BANAL-20-116 (NSPAAR) you could generate by a template switch orcopy-choice error a virus with something very close to NSPRRARS. It's very possible.
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So Yuri - the masterblaster - got that part right about the reverse complement (his pango mRNA is the reverse complement), That being said - I am a little biased toward most of the copy choice errors involving mixed virus infections for the reason that the replication machinery for the virus is complicated by the fact that you need thenucleocapsid proteins and other factors bound to the template to get efficient replication "transcription" ( the pos toneg first round or if you a re a neg strand virus the first neg to pos round). Does not mean it can't involve a mRNA -Yuri even found some examples in the literature - just means for me it's much more infrequent.
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https://journals.asm.org/doi/10.1128/JVI.79.11.6620-6630.2005
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2021-10-03 07:49
Robert F. Garry
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908549/
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The old schoolers call the first round "replication" (generates the replicative intermediates). The second round they call "transcription"- generates the mRNAs.
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Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs
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Also I have always considered transcription to be DNA->RNA?
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@Robert Garry The 'second round' takes place in the ER so might be more likely to have lots of other templates toswitch too, I guess. But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.