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Slack / Private Message Drop, pp.1013-1014 [SLACK_001227] · slack_pm:msg:10585

Page text: p.1013, p.1014 · original PDF

Date: 2021-10-03 08:09
Type: chat message · slack
recipient: Kristian G. Andersen, Robert F. Garry, Edward C. Holmes
speaker: Andrew Rambaut
Topics: Vaccines

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Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs

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Statement	Grade	Attribution	Stance
Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs	needs context	uncertain explicit hedge	asserts

Statement

Grade

Attribution

Stance

Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs

needs context

uncertain explicit hedge

asserts

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2021-10-03 07:29 Robert F. Garry open

So Yuri - the masterblaster - got that part right about the reverse complement (his pango mRNA is the reverse complement), That being said - I am a little biased toward most of the copy choice errors involving mixed virus infections for the reason that the replication machinery for the virus is complicated by the fact that you need thenucleocapsid proteins and other factors bound to the template to get efficient replication "transcription" ( the pos toneg first round or if you a re a neg strand virus the first neg to pos round). Does not mean it can't involve a mRNA -Yuri even found some examples in the literature - just means for me it's much more infrequent.
2021-10-03 07:46 Robert F. Garry open

https://journals.asm.org/doi/10.1128/JVI.79.11.6620-6630.2005
2021-10-03 07:49 Robert F. Garry open

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908549/
2021-10-03 07:54 Robert F. Garry open

The old schoolers call the first round "replication" (generates the replicative intermediates). The second round they call "transcription"- generates the mRNAs.
2021-10-03 08:09 Andrew Rambaut

Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs
2021-10-03 08:09 Andrew Rambaut open

Also I have always considered transcription to be DNA->RNA?
2021-10-03 08:23 Andrew Rambaut open

@Robert Garry The 'second round' takes place in the ER so might be more likely to have lots of other templates toswitch too, I guess. But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.
2021-10-03 09:04 Robert F. Garry open

@Andrew Rambaut Agree - I don't like the terminology either - usually just say + to - or - to +. and for a positive virusof course as you say - to + can also be creating genomes so "replication" in my mind.
2021-10-03 09:05 Robert F. Garry open

Yes indeed - lots of the accessory proteins are just so the replicative intermediates can associate with membrane.

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