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Slack / Private Message Drop, p.1014 [SLACK_001228] · slack_pm:msg:10588

Date: 2021-10-03 09:04
Type: chat message · slack
mentioned: Andrew Rambaut
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry

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@Andrew Rambaut Agree - I don't like the terminology either - usually just say + to - or - to +. and for a positive virusof course as you say - to + can also be creating genomes so "replication" in my mind.

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A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2021-10-03 07:54 Robert F. Garry open

The old schoolers call the first round "replication" (generates the replicative intermediates). The second round they call "transcription"- generates the mRNAs.
2021-10-03 08:09 Andrew Rambaut open

Although with all the sub-genomic mRNAs being created in the second round perhaps it is worth distinguishing the actual production of the gRNA from mRNAs
2021-10-03 08:09 Andrew Rambaut open

Also I have always considered transcription to be DNA->RNA?
2021-10-03 08:23 Andrew Rambaut open

@Robert Garry The 'second round' takes place in the ER so might be more likely to have lots of other templates toswitch too, I guess. But first round will be way more consequential as all progeny would have the insert and the spike proteins would have the FCS.
2021-10-03 09:04 Robert F. Garry

@Andrew Rambaut Agree - I don't like the terminology either - usually just say + to - or - to +. and for a positive virusof course as you say - to + can also be creating genomes so "replication" in my mind.
2021-10-03 09:05 Robert F. Garry open

Yes indeed - lots of the accessory proteins are just so the replicative intermediates can associate with membrane.

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