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Slack / Private Message Drop, p.523 [SLACK_000737] · slack_pm:msg:05453

Date: 2021-02-17 16:37
Type: chat message · slack
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry

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Q677P - highly likelihood of 3/3 sites O-glycosylated in mucosal tissues. Q677H - only 2/3 sites with O-glycans -moderate probability that sites are used in mucosal tissues. 681R and H also knock down to only 2/3 sites with O-glycans - moderate probability that sites are used in mucosal tissues. Maybe "epistasis" going on with mutations like DouG that loosen-up the trimer and make it more "cleavable" - Maybe some of the FCS mutations tuning up the spot for optimum efficiency and timing of cleavage (like Goldilock's soup - not too hot not too cold).

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2021-02-17 16:15 Robert F. Garry open

It will be interesting to see what the Q677P mutation does to the O-glycans - it actually jacks up the prediction for each of the 3 sites quite a bit.
2021-02-17 16:18 Andrew Rambaut open

And 681R removes the O-glycan and makes it more basic?
2021-02-17 16:18 Andrew Rambaut open

How would you rank 677P, 681R and 681H?
2021-02-17 16:37 Robert F. Garry

Q677P - highly likelihood of 3/3 sites O-glycosylated in mucosal tissues. Q677H - only 2/3 sites with O-glycans -moderate probability that sites are used in mucosal tissues. 681R and H also knock down to only 2/3 sites with O-glycans - moderate probability that sites are used in mucosal tissues. Maybe "epistasis" going on with mutations like DouG that loosen-up the trimer and make it more "cleavable" - Maybe some of the FCS mutations tuning up the spot for optimum efficiency and timing of cleavage (like Goldilock's soup - not too hot not too cold).
2021-02-17 17:09 Andrew Rambaut open

Thanks!
2021-02-17 17:11 Kristian G. Andersen open

681R is interesting in this regard - might lose some control over the cleavage, but overall make it more 'cleavable'?

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