Private channel session 991

6 messages over 55m, 2021-02-17 – 2021-02-17.

A “conversation” here is an activity session — a run of messages with under 60 minutes of silence inside it. The channel had no native conversation boundaries.

1. 2021-02-17 16:15 Robert F. Garry open PDF p.523
   It will be interesting to see what the Q677P mutation does to the O-glycans - it actually jacks up the prediction for each of the 3 sites quite a bit.
2. 2021-02-17 16:18 Andrew Rambaut open PDF p.523
   And 681R removes the O-glycan and makes it more basic?
3. 2021-02-17 16:18 Andrew Rambaut open PDF p.523
   How would you rank 677P, 681R and 681H?
4. 2021-02-17 16:37 Robert F. Garry open PDF p.523
   Q677P - highly likelihood of 3/3 sites O-glycosylated in mucosal tissues. Q677H - only 2/3 sites with O-glycans -moderate probability that sites are used in mucosal tissues. 681R and H also knock down to only 2/3 sites with O-glycans - moderate probability that sites are used in mucosal tissues. Maybe "epistasis" going on with mutations like DouG that loosen-up the trimer and make it more "cleavable" - Maybe some of the FCS mutations tuning up the spot for optimum efficiency and timing of cleavage (like Goldilock's soup - not too hot not too cold).
5. 2021-02-17 17:09 Andrew Rambaut open PDF p.523
   Thanks!
6. 2021-02-17 17:11 Kristian G. Andersen open PDF p.524
   681R is interesting in this regard - might lose some control over the cleavage, but overall make it more 'cleavable'?