Slack / Private Message Drop — page 523

leads to the second [2021-02-17 14:34:58] [Eddie Holmes] As for Laura Boykin - idiotic nonsense. I'm not sure how B.1.1.7 counts as white. [2021-02-17 14:35:24] [Andrew Rambaut] I think she is confused. [2021-02-17 14:35:40] [Eddie Holmes] @Robert Garry I've gone through the text again Bob - looks good. I'm happy with the cuts. [2021-02-17 14:37:18] [Robert Garry] THANKS Eddie _ I'll clean it up a bit more for Andrew and Kristian... [2021-02-17 14:44:09] [Kristian Andersen] [thread - ID: 2021-02-17 14:33:35] @Andrew Rambaut Very clear... Glad she had a chance to teach you how to do it properly. [2021-02-17 14:58:45] [Robert Garry] https://www.biorxiv.org/content/10.1101/2021.02.05.429982v1 [2021-02-17 14:59:22] [Kristian Andersen] Ohhhhhh, we rock SO much! :boom: [2021-02-17 16:15:19] [Robert Garry] It will be interesting to see what the Q677P mutation does to the O-glycans - it actually jacks up the prediction for each of the 3 sites quite a bit. [2021-02-17 16:18:21] [Andrew Rambaut] And 681R removes the O-glycan and makes it more basic? [2021-02-17 16:18:52] [Andrew Rambaut] How would you rank 677P, 681R and 681H? [2021-02-17 16:37:16] [Robert Garry] Q677P - highly likelihood of 3/3 sites O-glycosylated in mucosal tissues. Q677H - only 2/3 sites with O-glycans - moderate probability that sites are used in mucosal tissues. 681R and H also knock down to only 2/3 sites with O-glycans - moderate probability that sites are used in mucosal tissues. Maybe "epistasis" going on with mutations like DouG that loosen-up the trimer and make it more "cleavable" - Maybe some of the FCS mutations tuning up the spot for optimum efficiency and timing of cleavage (like Goldilock's soup - not too hot not too cold). [2021-02-17 17:09:11] [Andrew Rambaut] Thanks!