Chat message

Slack / Private Message Drop, p.524 [SLACK_000738] · slack_pm:msg:05455

Recipients on this medium are inferred from channel membership, not per-message addressing.

681R is interesting in this regard - might lose some control over the cleavage, but overall make it more 'cleavable'?

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2021-02-17 16:18 Andrew Rambaut open

And 681R removes the O-glycan and makes it more basic?
2021-02-17 16:18 Andrew Rambaut open

How would you rank 677P, 681R and 681H?
2021-02-17 16:37 Robert F. Garry open

Q677P - highly likelihood of 3/3 sites O-glycosylated in mucosal tissues. Q677H - only 2/3 sites with O-glycans -moderate probability that sites are used in mucosal tissues. 681R and H also knock down to only 2/3 sites with O-glycans - moderate probability that sites are used in mucosal tissues. Maybe "epistasis" going on with mutations like DouG that loosen-up the trimer and make it more "cleavable" - Maybe some of the FCS mutations tuning up the spot for optimum efficiency and timing of cleavage (like Goldilock's soup - not too hot not too cold).
2021-02-17 17:09 Andrew Rambaut open

Thanks!
2021-02-17 17:11 Kristian G. Andersen

681R is interesting in this regard - might lose some control over the cleavage, but overall make it more 'cleavable'?