Slack / Private Message Drop — page 186

Rupert's take down of that poll was brilliant! F Bollocks is such an arse - unbelievable. https://twitter.com/bealelab/status/1307437018645958658?s=21 [2020-09-20 05:39:02] [Robert Garry] Yes great job Kristian - unfortunately necessary. Kudos also for proper use of the word hooey in a tweet and good response to GVN. [2020-09-20 06:52:02] [Robert Garry] ```ppttssrlrraTsg``` spike glycoprotein [BtPa-BetaCoV/GD2013] GenBank: AIA62343.1 This one is from another Merbecovirus a little closer to where it might have mattered in China. Bottom line, and maybe it's my myopia for not realizing this before, but there are bat betacoronaviruses in China that have furin cleavage sites that evolved naturally. This one also has a predicted O-glycan, which may or may not be used. [2020-09-20 06:54:56] [Robert Garry] pptistrlrratSgvS spike [Bat coronavirus (BtCoV/A434/2005)] GenBank: ABG11962.1 Here's the other onefrom china - two predicted O-glycans [2020-09-20 06:57:32] [Robert Garry] https://virological.org/t/the-sarbecovirus-origin-of-sars-cov-2-s-furin-cleavage-site/536 makes this conversation a bit more relevant - although that virological post seems to have been one of the factors that sent Alina down the rabbit hole. [2020-09-20 07:45:50] [Robert Garry] ppttsTrvrraTSgvsS spike glycoprotein [Pipistrellus abramus bat coronavirus HKU5-related] GenBank: QHA24687.1 best for last FOUR predicted O-glycans. [2020-09-20 14:04:14] [Eddie Holmes] Yes, I saw that one. Those furin cleavage sites are definitely out there in nature. [2020-09-20 15:28:16] [Robert Garry] The other common thread is that when they appear they are generally insertions: Here's HKU5 S aligned with closely related HKU4 S Merbecoviruses. Pattern repeats with HKU1, OC43 (Embecoviruses]. SARS2 vs the other Sarbecoviruses is showing it can happen with the Sarbecoviruses. [shared file(s): image.png] [2020-09-20 17:31:09] [Eddie Holmes] That's really interesting! [2020-09-20 17:35:44] [Eddie Holmes] Is that a furin insertion? [2020-09-20 18:57:06] [Kristian Andersen] Bob, time for your first Virological post... this is important :wink: