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Slack / Private Message Drop, p.1003 [SLACK_001217] · slack_pm:msg:10468

Date: 2021-09-21 17:14
Type: chat message · slack
mentioned: Robert F. Garry
recipient: Robert F. Garry, Edward C. Holmes, Andrew Rambaut
speaker: Kristian G. Andersen
Topics: Furin cleavage site and molecular features

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@Robert Garry what are the key N-linked sites for the DC-SIGN/L-SIGN stuff? I have my eye focused on residue 370 in SC2 (357 in SC1) since that is a FULLY conserved N-linked glycan across _all_ sarbecos, _except_ in SC2 because residue 372 changes from a fully conserved T to an A (via a single A>G transition in the first codon position). In the RBD there are three N-linked glycans in SC2/BANAL, so two are conserved, one is not.

Extracted statements

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Statement	Grade	Attribution	Stance
In the RBD there are three N-linked glycans in SC2/BANAL, so two are conserved, one is not.	own voice, substantive	speaker_own	asserts

Statement

Grade

Attribution

Stance

In the RBD there are three N-linked glycans in SC2/BANAL, so two are conserved, one is not.

own voice, substantive

speaker_own

asserts

In context

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2021-09-21 16:48 Kristian G. Andersen open

It's not "PRRAR", that's for sure.
2021-09-21 16:48 Kristian G. Andersen open

@Robert Garry - I'm using NetNGlyc and since N-linked glycans are easy to predict I think I have what I need
2021-09-21 16:51 Robert F. Garry open

for sc1 the ones I have my eye on are N 29, 73 and maybe 227
2021-09-21 16:52 Robert F. Garry open

variable with close bat viruses like KY417150
2021-09-21 17:14 Kristian G. Andersen

@Robert Garry what are the key N-linked sites for the DC-SIGN/L-SIGN stuff? I have my eye focused on residue 370 in SC2 (357 in SC1) since that is a FULLY conserved N-linked glycan across _all_ sarbecos, _except_ in SC2 because residue 372 changes from a fully conserved T to an A (via a single A>G transition in the first codon position). In the RBD there are three N-linked glycans in SC2/BANAL, so two are conserved, one is not.
2021-09-21 17:19 Kristian G. Andersen open

We have _some_ information about what this particular residue does in SC2: https://pubmed.ncbi.nlm.nih.gov/34289344/

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