Slack / Private Message Drop — page 1003

[2021-09-21 16:25:21] [Robert Garry] SC1 coming [2021-09-21 16:37:56] [Eddie Holmes] I'd love to know what a 'human specific furin cleavage site' is. [2021-09-21 16:48:28] [Kristian Andersen] It's not "PRRAR", that's for sure. [2021-09-21 16:48:58] [Kristian Andersen] @Robert Garry - I'm using NetNGlyc and since N-linked glycans are easy to predict I think I have what I need [2021-09-21 16:51:41] [Robert Garry] for sc1 the ones I have my eye on are N 29, 73 and maybe 227 [2021-09-21 16:52:49] [Robert Garry] variable with close bat viruses like KY417150 [2021-09-21 17:14:25] [Kristian Andersen] @Robert Garry what are the key N-linked sites for the DC-SIGN/L-SIGN stuff? I have my eye focused on residue 370 in SC2 (357 in SC1) since that is a FULLY conserved N-linked glycan across _all_ sarbecos, _except_ in SC2 because residue 372 changes from a fully conserved T to an A (via a single A>G transition in the first codon position). In the RBD there are three N-linked glycans in SC2/BANAL, so two are conserved, one is not. [2021-09-21 17:19:19] [Kristian Andersen] We have _some_ information about what this particular residue does in SC2: https://pubmed.ncbi.nlm.nih.gov/34289344/ [2021-09-21 18:24:13] [Eddie Holmes] MHV has RRAR. FCoV has PRRAR. Would they have used the FCS's from these (although different codons)? I note that these viruses been used by groups in Wuhan in a different context: [2021-09-21 18:24:44] [Eddie Holmes] shared file(s): JVI.00948-15.pdf [2021-09-22 14:05:51] [Andrew Rambaut] I think we that maybe we could have a chat tomorrow about the Baric/Daszak shitshow? I would just like to discuss what our strategy should be so we don't get associated with these fuckers. [2021-09-22 14:11:56] [Kristian Andersen] Yeah, I think that would be good - we can't ignore this and I also need to get something back to Tony's team at NIH.