A single line often inverts meaning once you see what it
answers, so neighbouring messages are always shown.
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And if you just out sampling to look for diversity you are probably going to store the samples to preserve RNA rather than hope to culture anything.
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Yes - you might try to get lucky and if your cold chain was good freeze some sample away, but RNA Is the way to goin most field situations.
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But - most likely - screening by culturing is not going to be a sensitive as screening the RNA, if you know what you are doing with the RNA .
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We and colleagues do this all the time. The plan is always to get into a cold chain as quick as possible for sequencing. We do have a portable -80C we use in some cases, but it's usually a case of storage in RNAlater.
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2021-03-08 15:02
Robert F. Garry
In general the RNA (even fragmented non-infectious) hangs a lot longer than any infectious virus.
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Yeah, culturing viruses from bats or any other animal = bloody hard. I wouldn't be surprised that if you had 50 bats all infected with SC2, you'd only be able to grow it out of one of those.
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Explains some of the problems in the diagnostic space - Start mixing in detection with antigen assays [which correlates not so well with infectious virus or RNA] and it gets complicated fast.
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But that means these ideas about WIV sampling viruses from the wild and one of the accidentally escapes might bewrong?
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I think most of those people underestimate how incredibly hard it is to grow stuff from such samples, yes.