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1. 2021-09-21 08:30 Kristian G. Andersen open
   Well, in the grant they specifically talk about *new* viruses - so a SC2 precursor could have been one of those "low risk" viruses they talk about and then they started fiddling with it.
2. 2021-09-21 08:34 Andrew Rambaut open
   They would have selected it from candidates by genome sequencing it. I guess they would have seen an ACE-2 binding RBD and thought that is the one to try an FCS in?
3. 2021-09-21 08:37 Andrew Rambaut open
   Or they just were selecting viruses by seeing if they could get any to culture and then worry about genome sequencing?
4. 2021-09-21 08:48 Kristian G. Andersen open
   The former - which is the approach they describe in the grant.
5. 2021-09-21 08:49 Kristian G. Andersen
   Culture is very challenging so I don't think that approach makes sense.
6. 2021-09-21 09:00 Kristian G. Andersen open
   Here's what I suggest we do: • Make (spike) alignments with SC1, SARSr-CoVs, RaTG13(+close friends), Pangos, BANAL, SHC014, rs4237, SC2 • Mark 'key' sites based on what's in the grant - @Robert Garry, can you please help highlight the N-linked glycans based on Wuhan Hu-1 coordinates• Check to see if there's anything odd between SC2, basal viruses, and SC1• Look at e.g., codon usage at those sites if anything stands out• Cross-check with how those sites have evolved during the pandemic
7. 2021-09-21 09:01 Andrew Rambaut open
   And stay off email
8. 2021-09-21 09:02 Andrew Rambaut open
   Or carefully curate some emails for the FOI records
9. 2021-09-21 09:07 Robert F. Garry open
   OK I'm on my bit...

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