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Slack / Private Message Drop, p.700 [SLACK_000914] · slack_pm:msg:07342

Page text: p.700 · original PDF

Date
2021-04-13 14:52
Type
chat message · slack
recipient
Kristian G. Andersen, Robert F. Garry, Andrew Rambaut
speaker
Edward C. Holmes
Topics
Natural origin / zoonotic spillover

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Intermediate host has a complex bat-pangolin recombinant?

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Intermediate host has a complex bat-pangolin recombinant? needs context speaker_own questions

In context

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  1. 2021-04-13 14:45 Andrew Rambaut open
    So 69-70 deletion and the 144 deletion provide greater infectivity (and presumably when combined with 501Y RBD and 681H furin site) result in higher transmissibility.
  2. 2021-04-13 14:49 Andrew Rambaut open
    Other thing I got today was that the 3 nucleotide mutation in the middle of N that arose in the early Italian lineage probably create a new sgRNA - the resulting nucleotide sequence is complementary to the sgRNA transcriptionleader at the 5' end. That 3 nucleotide mutation had been bugging me for ages.
  3. 2021-04-13 14:51 Andrew Rambaut open
    The cool thing is that explains the N:D3L (also a 3 nucleotide mutation) in B.1.1.7 - Sheffield showed this probably enhances the recombination with the leader two - so it may be compensating to create more N
  4. 2021-04-13 14:52 Andrew Rambaut open
    Had a lovely afternoon of geeky genomic virology.
  5. 2021-04-13 14:52 Edward C. Holmes
    Intermediate host has a complex bat-pangolin recombinant?
  6. 2021-04-13 14:55 Andrew Rambaut open
    I think you could argue that the pangolin is actually less likely to be the intermediate for SC2. The original Wuhan was more bat like in its NTD and it took all the way to B.1.1.7 before it hit its stride (you also see the same mutationsin chronic infections - so again that might explain the fitness jump to get there).
  7. 2021-04-13 14:56 Andrew Rambaut open
    There could be other NTD point mutations that provide a similar effect.
  8. 2021-04-13 15:01 Edward C. Holmes open
    I think that's what I was I trying to say - that the intermediate host is a different species....but has a virus with acomplex recombinant history, like SIVcpz.
  9. 2021-04-13 15:04 Edward C. Holmes open
    Or that WIV weren't finished in their engineering job

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