Chat message

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

1. 2021-01-25 07:57 Robert F. Garry open
   @Kristian Andersen the R214C and G261C are *definitely* forming a new cysteine bridge. They are close enough in the structure that they WILL shift around to make the bridge. The existing disulfide bonds are C12 - C136, C131C166, C291 -C301. C12 is [apparently] the N -terminus and C12 to up to the orange A26 is "disordered" and therefore not in the structure I used to model spike. There is a newer structure (unstabilized cryo that gets the wholen terminus, including an N17) - I need to redo that models for sure putting in C12. I might even update some of the Virological Figures. Unpaired cysteines are *very rare* in viral glycoproteins. ALL the sarbecovirus spike cysteines are completely conserved from RmYN02, other Bat Covs, Pango Covs thru SARS-CoV to SC2. THIS IS HIGHLY UNUSUAL, BUT ULTRACOOL. Need to look at other CoVS to see the C-C structures in NTD. The role of NTD inSARS CoVs definately understudied. [shared file(s): image.png]
2. 2021-01-25 08:01 Robert F. Garry open
   Those two residues have or are next to a residues that's changed before. Opps didn't change the mink - it's G261D -the GoD mutation.
3. 2021-01-25 08:05 Robert F. Garry
   New sequences disprove conspiracy theories based on a laboratory contrived origin of bat coronavirus RatG13 and pangolin coronaviruses.

Full conversation →