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Slack / Private Message Drop, p.448 [SLACK_000662] · slack_pm:msg:04642

Date: 2021-01-25 07:57
Type: chat message · slack
mentioned: Kristian G. Andersen
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry

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@Kristian Andersen the R214C and G261C are *definitely* forming a new cysteine bridge. They are close enough in the structure that they WILL shift around to make the bridge. The existing disulfide bonds are C12 - C136, C131C166, C291 -C301. C12 is [apparently] the N -terminus and C12 to up to the orange A26 is "disordered" and therefore not in the structure I used to model spike. There is a newer structure (unstabilized cryo that gets the wholen terminus, including an N17) - I need to redo that models for sure putting in C12. I might even update some of the Virological Figures. Unpaired cysteines are *very rare* in viral glycoproteins. ALL the sarbecovirus spike cysteines are completely conserved from RmYN02, other Bat Covs, Pango Covs thru SARS-CoV to SC2. THIS IS HIGHLY UNUSUAL, BUT ULTRACOOL. Need to look at other CoVS to see the C-C structures in NTD. The role of NTD inSARS CoVs definately understudied. [shared file(s): image.png]

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2021-01-25 07:57 Robert F. Garry

@Kristian Andersen the R214C and G261C are *definitely* forming a new cysteine bridge. They are close enough in the structure that they WILL shift around to make the bridge. The existing disulfide bonds are C12 - C136, C131C166, C291 -C301. C12 is [apparently] the N -terminus and C12 to up to the orange A26 is "disordered" and therefore not in the structure I used to model spike. There is a newer structure (unstabilized cryo that gets the wholen terminus, including an N17) - I need to redo that models for sure putting in C12. I might even update some of the Virological Figures. Unpaired cysteines are *very rare* in viral glycoproteins. ALL the sarbecovirus spike cysteines are completely conserved from RmYN02, other Bat Covs, Pango Covs thru SARS-CoV to SC2. THIS IS HIGHLY UNUSUAL, BUT ULTRACOOL. Need to look at other CoVS to see the C-C structures in NTD. The role of NTD inSARS CoVs definately understudied. [shared file(s): image.png]
2021-01-25 08:01 Robert F. Garry open

Those two residues have or are next to a residues that's changed before. Opps didn't change the mink - it's G261D -the GoD mutation.
2021-01-25 08:05 Robert F. Garry open

New sequences disprove conspiracy theories based on a laboratory contrived origin of bat coronavirus RatG13 and pangolin coronaviruses.

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