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Slack / Private Message Drop, p.328 [SLACK_000542] · slack_pm:msg:03447

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@Robert Garry In SC1 it's T501 fwiw.

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2020-12-20 07:54 Robert F. Garry open

Benton et al. is going to be the key to interpreting the NTD deletions and probably the other elephant mutations aswell. A pretty good interpretation of D614G therein. More importantly it clear that *both* NTD and the RBD need toundergo dramatic shifts that are driven by ACE2 binding to accommodate the S1/S2 and S2' cleavages and ultimately the large scale rearrangement of S2 into the 6 helix bundle. This doesn't rule out an NTD "receptor." Ineed to get to the office to look at the structures in Benton in a bit more detail to see what del69-70 etc do to theBenton structures, but it won't surprise me if they don't "destabilize" the trimer ala Doug.
2020-12-20 07:59 Andrew Rambaut open

@Robert Garry I don't think 501Y turns up in mink.
2020-12-20 08:12 Robert F. Garry open

@Robert Garry It's N501T
2020-12-20 08:15 Robert F. Garry open

@Robert Garry Same residue changes to two diff amino acids when SC2 "adapts" to two diff animals.
2020-12-20 08:27 Robert F. Garry

@Robert Garry In SC1 it's T501 fwiw.
2020-12-20 08:33 Robert F. Garry open

Note to self that the Benton structures are "stabilized" with two proline substitutions - this is what we did to ultimately get the Lassa GP structure.