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Slack / Private Message Drop, p.54 [SLACK_000268] · slack_pm:msg:00541

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Eh? Don't they trim primers? iVar does that...

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2020-06-04 17:19 Andrew Rambaut open

It is my retirement project - become a creationist.
2020-06-04 17:20 Kristian G. Andersen open

Get the Templeton Prize > that's your ticket
2020-06-04 17:21 Andrew Rambaut open

It is really annoying - those BC sequences that sit between WA1 and WA2 cluster are almost certainly artefacts (the second defining mutation is in a primer site and they are calling it for the primer). Happens with some of the other BCones in the WA2 cluster too.
2020-06-04 17:21 Andrew Rambaut open

But the folk in the BCCDC won't do anything about it until they have checked it with sanger sequencing
2020-06-04 17:21 Kristian G. Andersen

Eh? Don't they trim primers? iVar does that...
2020-06-04 17:23 Andrew Rambaut open

I don't know the details. It was Nick that was discussing it with them.
2020-06-04 17:23 Kristian G. Andersen open

Hmmm. They certainly should. Does this affect the conclusions from your recent preprint?
2020-06-04 17:25 Andrew Rambaut open

We excluded them because we thought they were flawed. Some of the arguments are based on the fact there are no intermediates with only one of the two SNPs. But they are still there and of course the reviewers have pointed itout.
2020-06-04 17:25 Andrew Rambaut open

Anyway - not my problem (Mike's problem).