Slack / Private Message Drop — page 426

[2021-01-19 18:14:35] [Eddie Holmes] Fair point. [2021-01-19 18:20:36] [Kristian Andersen] Looking at that bat virus I'm honestly surprised we haven't all died of coronavirus infections already. Such a wild family. Interesting to see a partial 'human' RBD [2021-01-19 18:25:50] [Eddie Holmes] I'm mainly looking forward to Alina and crowd trying to take it down [2021-01-19 18:32:47] [Robert Garry] All the building blocks are in bats. But, I'm with Eddie in thinking that Pangos have a couple of related Pango virus lineages they are carrying - that's despite Daszak and Alina musing that the pangos get infected after they are captured by humans or some other captured animal. The pango that get caught are the sick ones - just like the monkeys/apes with Ebola virus are sick and get captured. The third pango cov from China pretty much seals it for me. [2021-01-19 18:35:56] [Eddie Holmes] Looking foward to Tommy's pango data...being sequenced now I think. [2021-01-19 18:36:40] [Eddie Holmes] Weifeng has written this review paper and stuck my name on it. It contains the following paragraphs: "On June 11, 2020, a COVID-19 resurgence was reported in Beijing after 56 consecutive days without confirmed local cases in Beijing. By mid-June, a total of 368 cases were confirmed and epidemiological investigation linked all the confirmed cases to the Xinfadi market, suggesting a single infection source. Genomic surveillance and epidemiological investigation identified the same SARS-CoV-2 virus from the frozen salmon internationally imported in the cold storage which sold salmon to a booth in the Xinfadi market. This outbreak highlights that cold-chain food contamination may be responsible for the resurgence in Beijing. After that China began to perform routine surveillance of SARS-CoV-2 for cold-chain related occupational population. Similarly, the source for the COVID-19 outbreak in Qingdao Municipal Chest hospital was identified to be two stevedores responsible for internationally imported seafood who tested positive for SARS-CoV-2 in a routine test on September 24, 2020. SARS-CoV-2 nucleic was detected in cold-chain food and environmental samples and live SARS-CoV-2 was also successfully isolated from xx [Zhangyong EID], which provided direct evidence that SARS-CoV-2 contaminated seafood is able to trigger human infections. It is worth mentioning that there were internationally and domestically imported seafood on sale in the Huanan seafood market before it was closed on January 1, 2020. Whether contaminated cold-chain food initiated the infections at the Huanan seafood market remains elusive." [2021-01-19 18:37:00] [Robert Garry] Author list? Opt 1 - switch Eddie and Kristian and make Eddie corresponding. Opt 2 leave Eddie at second and make him co-first. Not sure where to put Tommy in either case. [2021-01-19 18:37:17] [Eddie Holmes] "In addition, nucleic fragments of SARS-CoV-2 were also detected using PCR and sequencing approaches. For example, SARS-CoV-2 in wastewater in northern Italy using both nested RT-PCR targeting the ORF1ab region and real-rime RT-PCR targeting the RdRp region, and the earliest positive samples were dated back to December 18, 2020 in Milan and Turin [La Rosa G et al., Sci Total Environ]. Similarly, the detection of SARS-CoV-2 in wastewater samples was also reported using real-time RT-PCR targeting the N gene, with the archived sample collected in Spain on March 12, 2019 [Randazzo et al., Water research]. The SARS-CoV-2 RNA was also detected in a patient in France in December 2019 using two different PCR-based assays and his last international travel was to Algeria in August 2019 [Deslandes et al., IJAA]. However, none of the three studies reported the viral sequences from the