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Slack / Private Message Drop, p.1015 [SLACK_001229] · slack_pm:msg:10598

Date: 2021-10-05 14:59
Type: chat message · slack
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry
Topics: Furin cleavage site and molecular features

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So, I'm taking another look at the BANAL-20-50 virus. To be honest I'm starting to think that this is even more than asmoking gun. The BANAL-20-50 nucleoprotein is *99.3%* similar to SARS2 Wu-1 at the amino acid level - that 's 3amino acids in an approximately 400 amino acid protein. The BANAL-20-50 spike is *98.4%* similar to SARS2 Wu-1at the amino acid level, including the PRRA insert. The long 1ab and 1a polyproteins are pretty much the same story~98% identical [there is a 9 amino acid insertion in nsp3 - don't hold me to that] in SC2 relative to BANAL-20-50. Atleast at the amino acid level BANAL-20-50 is SARS-CoV-1.98.

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2021-10-05 14:59 Robert F. Garry

So, I'm taking another look at the BANAL-20-50 virus. To be honest I'm starting to think that this is even more than asmoking gun. The BANAL-20-50 nucleoprotein is *99.3%* similar to SARS2 Wu-1 at the amino acid level - that 's 3amino acids in an approximately 400 amino acid protein. The BANAL-20-50 spike is *98.4%* similar to SARS2 Wu-1at the amino acid level, including the PRRA insert. The long 1ab and 1a polyproteins are pretty much the same story~98% identical [there is a 9 amino acid insertion in nsp3 - don't hold me to that] in SC2 relative to BANAL-20-50. Atleast at the amino acid level BANAL-20-50 is SARS-CoV-1.98.
2021-10-05 15:03 Edward C. Holmes open

Thanks Bob. So where does BANAL-20-50 differ most from SC2?
2021-10-05 15:09 Kristian G. Andersen open

Yes. These viruses are about as far from banal as can be...
2021-10-05 15:18 Robert F. Garry open

Not anywhere that I can see at least at the amino acid level. All of the proteins I've looked at so far are very very similar. Just looking at the proteins, you would really be hard pressed to call this a different virus species IMO. I'm guessing that most of the nt changes are third base changes (codon usage).
2021-10-05 15:37 Robert F. Garry open

From the paper - I think most of that minor orf3a difference is the 9 aa indel. [shared file(s): image.png]

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