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Slack / Private Message Drop, p.338 [SLACK_000552] · slack_pm:msg:03559

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Probably more 'immune' related than 'mouse' related?

In context

A single line often inverts meaning once you see what it answers, so neighbouring messages are always shown.

2020-12-22 07:29 Robert F. Garry open

From Sun - Fig S8 Shows that N501Y comes up quick followed by Q493H. then K417N. [shared file(s): image.png]
2020-12-22 07:40 Kristian G. Andersen

Probably more 'immune' related than 'mouse' related?
2020-12-22 07:48 Robert F. Garry open

Yeah - the fact these are epitopes to me strongly suggests immune selection - mouse or human. But I think that the particular substitution may depend on the species. Analysis coming...:sunglasses:
2020-12-22 08:11 Robert F. Garry open

https://jvi.asm.org/content/82/14/6984 Relevant paper: "They show that the major species barriers are determinedby interactions between four ACE2 residues (residues 31, 35, 38, and 353) and two RBD residues (residues 479and 487), that early civet SARS-CoV isolates were prevented from infecting human cells due to imbalanced saltbridges at the hydrophobic virus/receptor interface, and that SARS-CoV has evolved to gain sustained infectivity for human cells by eliminating unfavorable free charges at the interface through stepwise mutations at positions 479and 487."
2020-12-22 08:28 Robert F. Garry open

The equivalent amino acids to SC1 479 and 487 in terms of alignment to RBD appear to me to be 493 and 501, but Ineed to really take a closer look at ALL the structures.
2020-12-22 08:52 Robert F. Garry open

Those are the same and it's the alignment in PO1.