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Slack / Private Message Drop, pp.38-39 · slack_pm:msg:00374

Date: 2020-05-25 10:10 (hour precision)
Type: chat message · slack
mentioned: Kristian G. Andersen
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry
Topics: Intelligence community assessments

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@Kristian Andersen I'd just take this as more evidence for the short insertion hypothesis. A random event. The 12base pair insertion is - as ProfBill pointed out - out of frame. So if the insertion of the RR was purposeful why also create a proline insertion and put the 12 bases in out-of-frame? Not sure I buy that the "terminal G would have beenpurified out." Even with a 3X higher substitution rate don't most of the isolates still have CGGCGG after passage inhumans for at least 6 months?

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Statement	Grade	Attribution	Stance
The 12base pair insertion is - as ProfBill pointed out - out of frame.	own voice, substantive	speaker_own	asserts

Statement

Grade

Attribution

Stance

The 12base pair insertion is - as ProfBill pointed out - out of frame.

own voice, substantive

speaker_own

asserts

In context

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2020-05-25 10:10 Andrew Rambaut open

@kristian Andersen Doesn't Bill's observation of the acquisition of the insertion from another bat coronavirus explain the exact sequence of this and what codons it uses.
2020-05-25 10:10 Kristian G. Andersen open

@kristian Andersen Not quite sure if it does - CGG is part of the sequence Bill mentions, but not quite sure if CGG has to be conserved there. Need to check what those FIPs have at that site
2020-05-25 10:10 Kristian G. Andersen open

@kristian Andersen Curiously, they have exactly the same polybasic site, which I previously missed - also on Virological :wink:
2020-05-25 10:10 Kristian G. Andersen open

@kristian Andersen Here's what it looks like on the cat side - one CGG and one CGA. So no mystery really. [shared file(s): Screen Shot 2020-05-25 at 11.00.58 AM.png]
2020-05-25 10:10 Robert F. Garry

@Kristian Andersen I'd just take this as more evidence for the short insertion hypothesis. A random event. The 12base pair insertion is - as ProfBill pointed out - out of frame. So if the insertion of the RR was purposeful why also create a proline insertion and put the 12 bases in out-of-frame? Not sure I buy that the "terminal G would have beenpurified out." Even with a 3X higher substitution rate don't most of the isolates still have CGGCGG after passage inhumans for at least 6 months?

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