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Slack / Private Message Drop, p.7 [SLACK_000221] · slack_pm:msg:00060

Date: 2020-05-02 13:32
Type: chat message · slack
recipient: Kristian G. Andersen, Edward C. Holmes, Andrew Rambaut
speaker: Robert F. Garry

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> Yeah - something along those lines may well have happened. More bat viruses with "insertions/deletions" in theS1/S2 junction have to be out there. MERS-CoV actually pretty close. Furthermore, I have a link to some CoV PCR-positive bat samples from the Amazon (the rainforest, not the on-line megastore) - assume you're interested? Yes passaging in Vero's with and without trypsin important - trying to get hands on the Vero-TMPRSS cell linehttps://www.pnas.org/content/pnas/117/13/7001.full.pdf The S2" site of importance as well.

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journal https://www.pnas.org/content/pnas/117/13/7001.full.pdf

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2020-05-02 12:37 Robert F. Garry open

https://www.sciencedirect.com/science/article/pii/S1097276520302641#bib10
2020-05-02 12:49 Kristian G. Andersen open

Let's get some of those furin inhibits online
2020-05-02 12:50 Kristian G. Andersen open

Speaking of furin - Bob, your old buddy is on the Virological again and it's a pretty nice theory for how the furin site was acquired in bats - just need to add a little extra divergence and there you have it:http://virological.org/t/tackling-rumors-of-a-suspicious-origin-of-ncov2019/384/3
2020-05-02 13:32 Robert F. Garry

> Yeah - something along those lines may well have happened. More bat viruses with "insertions/deletions" in theS1/S2 junction have to be out there. MERS-CoV actually pretty close. Furthermore, I have a link to some CoV PCR-positive bat samples from the Amazon (the rainforest, not the on-line megastore) - assume you're interested? Yes passaging in Vero's with and without trypsin important - trying to get hands on the Vero-TMPRSS cell linehttps://www.pnas.org/content/pnas/117/13/7001.full.pdf The S2" site of importance as well.

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