Private channel session 717

6 messages over 30m, 2020-12-23 – 2020-12-23.

A “conversation” here is an activity session — a run of messages with under 60 minutes of silence inside it. The channel had no native conversation boundaries.

1. 2020-12-23 07:24 Kristian G. Andersen open PDF p.342
   A lot of good stuff here Bob - thanks! And for Nelly, maybe we're too focused on spike alone...
2. 2020-12-23 07:27 Robert F. Garry open PDF p.342
   Right on cue - nonSpike protein nsp6 @Andrew Rambaut as predicted - the deletion is in a large external loop - very likely to impact nsp6 function or regulation!
3. 2020-12-23 07:28 Robert F. Garry open PDF p.342
   shared file(s): image.png
4. 2020-12-23 07:36 Robert F. Garry open PDF p.342
   TM domain 4 has the seemingly odd arginine. This is a sign of dimerization. That and the fact that the loop connectsto this TM domain raises the suspicion these are part of the business/regulatory bits of the protein.
5. 2020-12-23 07:48 Robert F. Garry open PDF p.342
   AS for S477N - it would not surprise me to find that it's a contact residue with ACE2 - the ACE2 structures lack the nterminus (blue arrow) so plenty of opportunity for interactions.
6. 2020-12-23 07:54 Robert F. Garry open PDF p.342
   S477 [shared file(s): image.png]