Slack / Private Message Drop — page 863

spike W152C that creates an unpaired cysteine. This cysteine in the N-terminal domain of B.1.427/429 spike may coordinate with H69, H146 and H245 to bind a metal ion. RatG15 and RsYN04 have 3 or 4 predicted O-linked glycans near the S1/S2 junction (Indel 8). We note incongruence between the ORF1ab and Spike gene phylogenies of the SARS-CoV-2 related viruses suggesting widespread recombination. This is discussed in more detail in Zhou et al. (2021). *Figure 1 updated*. Amino acid alignment of the spike protein sequences of Cambodian, Thai Japanese and Chinese bat coronaviruses and other sarbecoviruses. The signal peptide, S1 subunit and part of S2 are shown. Positions of selected substitutions or deletions in 'variants of concern' are indicated. Added references: Li L-L, Wang J-L, Ma X-H, Li J-S, Yang X-F, Shi W-F and Duan Z-J. (2021). A novel SARS-CoV-2 related virus with complex recombination isolated from bats in Yunnan province, China bioRxiv 2021.03.17.435823; doi: https://doi.org/10.1101/2021.03.17.435823 Guo H, Hu B, Si H-R, Zhu Y, Zhang W, Li B, Li A, Geng R, Lin H-F, Yang X-L, Zhou P and Shi Z-L. (2021). Identification of a novel lineage bat SARS-related coronaviruses that use bat ACE2 receptor. bioRxiv 2021.05.21.445091; doi: https://doi.org/10.1101/2021.05.21.445091 Zhou H, Ji J, Chen X, Bi Y, Li J, Hu T, Hao Song H, Chen Y, Cui M, Zhang Y, Hughes AC, Holmes EC and Shi W. (2021).Identification of novel bat coronaviruses sheds light on the evolutionary origins of SARS-CoV-2 and related viruses. bioRxiv 2021.03.08.434390; doi: https://doi.org/10.1101/2021.03.08.434390 (Cell, in press). [shared file(s): image.png, image.png] [2021-05-24 15:51:46] [Kristian Andersen] On my phone. Bob, can you please share the link [2021-05-24 15:51:47] [Robert Garry] https://docs.google.com/document/d/1jmvbeZ1_RqQedPmfNKNs7DA_Z9sySsEWgAgZoVKRSWg/edit [2021-05-24 17:12:59] [Andrew Rambaut] Oh, Tami: https://twitter.com/conTaminatedsci/status/1396981846169341954 [2021-05-24 17:19:28] [Eddie Holmes] What a pillock [2021-05-24 17:26:15] [Kristian Andersen] In other news - this is NOT what I want to read. Note, epitopes are located in regions of the NTD (~215 / ~65) where we frequently see SARS-CoV-2 mutations. This could get fun. https://www.cell.com/cell/fulltext/S0092-8674(21)00662-0 [2021-05-24 18:22:07] [Andrew Rambaut] That is enough Twitter for tonight. Also been through the letter now. [2021-05-24 18:42:40] [Kristian Andersen] Hah, I can imagine - great work battling the idiots! Good practice for the letter... :wink:. I'll go through shortly.