Reading Room Production — page 375

From: "Carole Heilman" </O=NIH/OU=NIAID/CN=RECIPIENTS/CN=CHEILMAN> To: "Fauci, Anthony (NIH/NIAID)" <AFAUCI@niaid.nih.gov>, "La Montagne, John (NIH/NIAID)" fc: nih.gov>, "Killen, Jack (NIH/NCCAM)" EE omail.nin.gov> Cc: "McInnes, Pamela (NIH/NIAID)" EE @ viaia. nit. gov> Subject: Ectromelia/IL-4 - repeat Date: Tue, 16 Jul 2002 16:14:20 -0400 Importance: Normal Tony: Mark Buller has been working on repeating the Australian experiment that demonstrated that insertion of the IL4 gene in ectromelia resulted in a more virulent form. I wanted to update you with respect to these results. * He has successfully repeated these studies ¢ Prior vaccination with an ectromelia vaccine, however, protected the mice equally well from this new virulent virus when compared to WT ectromelia.. * However, prior vaccination with the related orthopox virus, vaccinia, protected only against ectromelia challenge and NOT against the ectromelia-IL4 construct. * The mechanism of the break-through immunity associated with the IL-4 insert is being further evaluated but appears to be mediated through the IL4 receptor, as Balb-C-IL-4 knockout mice appear to be protected under the heterologous challenge scenario Carole