Reading Room Production — page 106

Best regards, Zhengli, aa RAR E----- 4 X:Bloom PhD, Jesse D" irene AGE ATTA: 2022-03-12 01:53:51 (2 HA7N) WE: " wh.iov.cn" wh.iov.cn>, owt iov.cn" EE owh.iov.cn> PK: =u: RaTG13 sequence? Hi Zhengli and Peng, I was just reading a new interview with Peter Daszak (https://theintercept.com/2022/03/1 1/covid-nih-ecohealth-peter-daszakinterview/). In it, he says there are parts of the RaTG13 genome that "aren't correctly sequenced." I was curious if you knew what he meant, in particular which parts are incorrect if the RaTG13 genome indeed isn't correctly sequenced as Peter is saying. I was especially wondering if the errors might be in the spike? We've been using the RBDs in experiments, and finding it doesn't bind any bat ACE2s we have tested particularly well (see Figure 1b of https://www.nature.com/articles/s41586-02204464-z_reference.pdf). I was wondering if this could be affected by whatever sequencing problems Peter is referring to? Thanks, Jesse Jesse Bloom Professor, Fred Hutchinson Cancer Research Center Investigator, Howard Hughes Medical Institute