Reading Room Production — page 105

Professor, Fred Hutchinson Cancer Research Center Investigator, Howard Hughes Medical Institute From: GIES Mown.iov.cn> Date: Saturday, March 12, 2022 at 7:01 PM To: Bloom PhD, Jesse D Po tredhutch or > Ce: " own iov.cn>, fal HRS SIE Oh iov.on>, yee <@ecohealthalliance.org>, Peter Daszak ecohealthalliance.org> Subject: Re: Re: RaTG13 sequence? Sorry, I missed the email address of Peter. The TG13 sequence was not complete at its first publication, the missing parts are the 5' and 3' end. We are confident about our sequencing and you can find all the original data in the GenBank, including from NGS and PCR amplification sequencing. We submitted the original data later than the full-length genome, it's not because we wanted to hide something as someone speculated. I feel extremely sad to see those speculation regarding TG13. After COVIS-19, we have tried to work on the ACE2 utilization of TG13 with pseudovirus and binding assay and we have the same results with the published ones and yours. Recently, we have tried to work on recombinant virus based on WIV1 backbone, we couldn't rescue the WIV1-TG13-spike neither. That's mean the TG13 has a low binding affinity to human ACE2, that's the truth, the only truth! Best regards, Zhengli, << JRA BB E----- At X:"Bloom PhD, Jesse D" <p BC AY I] :2022-03-13 10:35:05 (HAA) WEA: "AE gg" wh.iov.cn> Pbk: "HARE" wh.iov.cn>, "Jal jis" {Eowh.iov.cn>, "25 }q) a8" @ecohealthalliance.org> Ew: Re: RaTG13 sequence? Hi Zhengli, I think you accidentally sent your message to me rather than to Peter Daszak. Anyway, here is a PDF of the article with the recent interview where he says RaTG13 isn't sequenced correctly. Maybe he just means the termini of the RaTG13 genome that you updated with recent RACE sequencing? If you have any insight, let me know as people are definitely asking a lot about this after reading his interview. Thanks, Jesse Jesse Bloom Professor, Fred Hutchinson Cancer Research Center Investigator, Howard Hughes Medical Institute From: 4iEFAN <Bibowh iov.cn> Date: Saturday, March 12, 2022 at 6:05 PM To: Gc: Bloom PhD, Jesse D Bo treahutch.org>, BAKE Bown iov.cn>, FalARS EE own iov.cn>, 2S io) ar ecohealthalliance.org> Subject: Fw: RaTG13 sequence? Hi, Peter, I got the email below. I'm not able to read the article. What do you mean regarding the sequencing issue?