Gates Package — page 847

Fig.6. -globin analysis after ex vivo base editing with by HDAd-EF1a.ABE8e. A) Percentage of -globin positive peripheral RBCs measured by flow cytometry at different time points after transplantation. N=5 animals. B) qRT-PCR for globin mRNA (human HBG/-globin vs mouse HBA/a-globin; human HBG/-globin vs mouse HBB/-globin; human HBG/-globin vs human HBB/-globin). Shown are the fold changes between untreated and treated animals. N=5 animals. C) Percentage of human −globin chains relative to mouse a−globin chains, mouse −globin, and human -globin chain measured by HPLC.