Gates Package — page 843

Fig.4. Cytospins from cultures at different time points of ED. A) Representative samples from Thal CD34+ cells, either untransduced or transduced with HDAd-EF1a.ABE8e and O6BG/BCNU selected. Left panel: cytospins stained with Giemsa/May-Grünwald. The arrows indicate cells at different stages of erythroid differentiation: proerythroblasts (red) → basophilic erythroblasts (green) →polychromatic erythroblasts (blue) →orthochromatic erythroblasts (orange) →maturing orthochromatic erythroblasts (yellow)→reticulocytes (black)/pyrenocytes (grey). The scale bar is 25mm. Right panel: Quantification of erythroid progenitors. Five random fields were counted by two scientists. *p<0.05. Pro: proerythroblasts, Baso: basophilic erythroblasts, Poly: polychromatic erythroblasts, Ortho: orthochromatic erythroblasts, mat. Ortho: maturing orthochromatic erythroblasts, Retics: reticulocytes, Pyrcs: pyrenocytes. B) Cytospins from differentiated SCD CD34+ cells at days 11, 14 and 18 of ED, untransduced and HDAd-EF1a.ABE8e-transduced without and with selection. Statistical analyses were performed using two-way ANOVA.