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RE: Matters Arising regarding your Nature paper

Reading Room Production, p.80 · reading_room:email:00083

Page text: p.80 · original PDF

Date
2021-11-24 19:51
Type
email · email
sender
Clare Thomas
Topics
Proximal Origin paperWuhan Institute of Virology collaboration
Dear Zhengli, Thanks again for providing very helpful responses to the Matters Arising. We do have an obligation to look into any comments about papers that we publish, particularly if they relate to concerns about insufficient methodological detail etc. I just wanted to ask you about a two more things, about the sample source, for the sake of completeness. The Matters Arising author points to a preprint that claims the raw data contain lower levels of bacterial reads than would be expected from a fecal swab. I cannot see from the methods anywhere that the nature of the sample was described. Was it a fecal swab? If so, is there an explanation for the low bacterial reads, if indeed that claim is true? And along the same lines, what was the sample used for the sequencing of the 5'end done in September 2020? Thanks in advance for clarifying these details, which do seem pertinent to the methods described in our paper and therefore we do have to ask. Once again I'm very sorry for having to trouble you on so many occasions with these queries. With best wishes, Clare

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Thanks in advance for clarifying these details, which do seem pertinent to the methods described in our paper and therefore we do have to ask. own voice, substantive speaker_own asserts

In context

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  1. 2021-11-24 09:10 unattributed open
    [External - Use Caution] Dear Clare, I'm sorry that you continue to recieve such emails. It's a pity that there are someone who is contining to doute and scruetine our work. This person has beeninterrupting me and my colleagues and want to find evidence to show that the SARS-CoV-2 is a lab leak. I don't think I should answer his unreasonable questions and crazy ideas. But for your refrence, I would like to send you more details although I think they are not whinth the duty of you and neither the scope of my publication in your journal. Best regards, Zhengli, Bown. iov.cn
  2. 2021-11-24 09:10 unattributed open
    Dear Clare, I'm sorry that you continue to recieve such emails. It's a pity that there are someone who is contining to doute and scruetine our work. This person has been interrupting me and my colleagues and want to find evidence to show that the SARS-CoV-2 is a lab leak. I don't think I should answer his unreasonable questions and crazy ideas. But for your refrence, I would like to send you more details although I think they are not whinth the duty of you and neither the scope of my publication in your journal. Best regards, Zhengli, Bown iov.cn
  3. 2021-11-24 09:10 unattributed open
    [External - Use Caution] Dear Clare, I'm sorry that you continue to recieve such emails. It's a pity that there are someone who is contining to doute and scruetine our work. This person has beeninterrupting me and my colleagues and want to find evidence to show that the SARS-CoV-2 is a lab leak. I don't think I should answer his unreasonable questions and crazy ideas. But for your refrence, I would like to send you more details although I think they are not whinth the duty of you and neither the scope of my publication in your journal. Best regards, Zhengli, Bown. iov.cn
  4. 2021-11-24 09:10 unattributed open
    Dear Clare, I'm sorry that you continue to recieve such emails. It's a pity that there are someone who is contining to doute and scruetine our work. This person has been interrupting me and my colleagues and want to find evidence to show that the SARS-CoV-2 is a lab leak. I don't think I should answer his unreasonable questions and crazy ideas. But for your refrence, I would like to send you more details although I think they are not whinth the duty of you and neither the scope of my publication in your journal. Best regards, Zhengli, Bown iov.cn
  5. 2021-11-24 19:51 Clare Thomas
    Dear Zhengli, Thanks again for providing very helpful responses to the Matters Arising. We do have an obligation to look into any comments about papers that we publish, particularly if they relate to concerns about insufficient methodological detail etc. I just wanted to ask you about a two more things, about the sample source, for the sake of completeness. The Matters Arising author points to a preprint that claims the raw data contain lower levels of bacterial reads than would be expected from a fecal swab. I cannot see from the methods anywhere that the nature of the sample was described. Was it a fecal swab? If so, is there an explanation for the low bacterial reads, if indeed that claim is true? And along the same lines, what was the sample used for the sequencing of the 5'end done in September 2020? Thanks in advance for clarifying these details, which do seem pertinent to the methods described in our paper and therefore we do have to ask. Once again I'm very sorry for having to trouble you on so many occasions with these queries. With best wishes, Clare
  6. 2021-11-24 19:51 Clare Thomas open
    Dear Zhengli, Thanks again for providing very helpful responses to the Matters Arising. We do have an obligation to look into any comments about papers that we publish, particularly if they relate to concerns about insufficient methodological detail etc. I just wanted to ask you about a two more things, about the sample source, for the sake of completeness. The Matters Arising author points to a preprint that claims the raw data contain lower levels of bacterial reads than would be expected from a fecal swab. I cannot see from the methods anywhere that the nature of the sample was described. Was it a fecal swab? If so, is there an explanation for the low bacterial reads, if indeed that claim is true? And along the same lines, what was the sample used for the sequencing of the 5'end done in September 2020? Thanks in advance for clarifying these details, which do seem pertinent to the methods described in our paper and therefore we do have to ask. Once again I'm very sorry for having to trouble you on so many occasions with these queries. With best wishes, Clare
  7. 2021-11-29 09:07 unattributed open
    [External - Use Caution] Dear Clare, Please see our answers as follows: The reasons for the low bacterial reads in the raw sequencing data of RaTG13 could be: 1) The sample is anal swab, not a fecal pellet. 2) We used the High Pure Viral RNA Kit for the RNA extraction. 3) The sequencing depth was not high. For the 5'race done in September 2020, we used the viral RNA previously extracted from that anal swab sample. Best regards, Zhengli, Bown. iov.cn From: Clare Thomas Date; 2021-11-24 19:51 To: wh.iov.cn Subject: RE: Matters Arising regarding your Nature paper Dear Zhengli, Thanks again for providing very helpful responses to the Matters Arising. We do have an obligation to look into any comments about papers that we publish, particularly if they relate to concerns about insufficient methodological detail etc. I just wanted to ask you about a two more things, about the sample source, for the sake of completeness. The Matters Arising author points to a preprint that claims the raw data contain lower levels of bacterial reads than would be expected from a fecal swab. I cannot see from the methods anywhere that the nature of the sample was described. Was it a fecal swab? If so, is there an explanation for the low bacterial reads, if indeed that claim is true? And along the same lines, what was the sample used for the sequencing of the 5'end done in September 2020? Thanks in advance for clarifying these details, which do seem pertinent to the methods described in our paper and therefore we do have to ask. Once again I'm very sorry for having to trouble you on so many occasions with these queries. With best wishes, Clare
  8. 2021-11-29 09:07 unattributed open
    [External - Use Caution] Dear Clare, Please see our answers as follows: The reasons for the low bacterial reads in the raw sequencing data of RaTG13 could be: 1) The sample is anal swab, not a fecal pellet. 2) We used the High Pure Viral RNA Kit for the RNA extraction. 3) The sequencing depth was not high. For the 5'race done in September 2020, we used the viral RNA previously extracted from that anal swab sample. Best regards, Zhengli, Bown. iov.cn From: Clare Thomas Date; 2021-11-24 19:51 To: wh.iov.cn Subject: RE: Matters Arising regarding your Nature paper Dear Zhengli, Thanks again for providing very helpful responses to the Matters Arising. We do have an obligation to look into any comments about papers that we publish, particularly if they relate to concerns about insufficient methodological detail etc. I just wanted to ask you about a two more things, about the sample source, for the sake of completeness. The Matters Arising author points to a preprint that claims the raw data contain lower levels of bacterial reads than would be expected from a fecal swab. I cannot see from the methods anywhere that the nature of the sample was described. Was it a fecal swab? If so, is there an explanation for the low bacterial reads, if indeed that claim is true? And along the same lines, what was the sample used for the sequencing of the 5'end done in September 2020? Thanks in advance for clarifying these details, which do seem pertinent to the methods described in our paper and therefore we do have to ask. Once again I'm very sorry for having to trouble you on so many occasions with these queries. With best wishes, Clare
  9. 2021-11-29 09:37 Clare Thomas open
    Dear Zhengli, Thank you very much. All the best, Clare

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