Slack / Private Message Drop — page 945

Yeah she's been emailing me to o. I expect drivel. :-1: [2021-06-16 19:24:55] [Eddie Holmes] Total drivel. As I noted before, Australia asked for an investigation into COVID origins in April 2020 so we can easily kick the 'we shutdown investigations' into touch. [2021-06-17 17:35:25] [Robert Garry] Long talk with Daszak today - PTSD - no one deserve what he been put thru - learned a few things about the WHO study. [2021-06-17 17:45:08] [Eddie Holmes] Indeed. One of my mantras has been how much worse it must be for Daszak. There's no way he is lying either. [2021-06-19 07:32:37] [Robert Garry] Well done on doc @Eddie Holmes @Andrew Rambaut almost there... [2021-06-19 08:59:44] [Andrew Rambaut] I have fixed a few minor things but will go over it tonight. Great work Eddie! [2021-06-19 10:10:18] [Robert Garry] Bloom - please read !channel IMPORTANT [shared file(s): paper.pdf] [2021-06-19 10:15:31] [Robert Garry] "However, the current study suggests that at least in one case, the trusting structures of science have been abused to obscure sequences relevant to the early spread of SARS-CoV-2 in Wuhan. A careful re-evaluation of other archived forms of scientific communication, reporting, and data could shed additional light on the early emergence of the virus." [2021-06-19 10:33:49] [Andrew Rambaut] Is it deep sequencing data? Or amplicons? [2021-06-19 10:35:40] [Andrew Rambaut] Amplicons - ```The study describes an approach to diagnose infection withSARS-CoV-2 and other respiratory viruses by nanopore sequenc-ing. This approach involved reverse-transcription of total RNAfrom swab samples, followed by PCR with specific primers togenerate amplicons covering portions of the viral genome. Theseamplicons were then sequenced on an Oxford Nanopore Grid-ION, and infection was diagnosed if the sequencing yieldedsufficient reads aligning to the viral genome. Importantly, thestudy notes that this approach yields information about thesequence of the virus as well enabling diagnosis of infection``` At least 2 of the mutations talked about are in or immediately adjacent to ARTIC primer sites which always ring a few alarm bells: ```C18060T MN908947.3 18036 18062 nCoV-2019_59_RIGHT 60 - C28144T MN908947.3 28145 28172 nCoV-2019_92_RIGHT 60 - G28085T