Reading Room Production — page 98

-----~ JSR RAB E----- BEX :"Bloom PhD, Jesse D" {il @fredhutch.org> AGE [A] :2022-03-13 10:35:05 (HAA) WEA: "4G 1E RN" <owh.iov.cn> Poik: "AA#=" Own iov.cn>, "JAS" Own iov.cn>, "2)4,38" <@ecohealthalliance.org> =a: Re: RaTG13 sequence? Hi Zhengli, I think you accidentally sent your message to me rather than to Peter Daszak. Anyway, here is a PDF of the article with the recent interview where he says RaTG13 isn't sequenced correctly. Maybe he just means the termini of the RaTG13 genome that you updated with recent RACE sequencing? If you have any insight, let me know as people are definitely asking a lot about this after reading his interview. Thanks, Jesse Jesse Bloom Professor, Fred Hutchinson Cancer Research Center Investigator, Howard Hughes Medical Institute From: 41EAN <lwh.iov.cn> Date: Saturday, March 12, 2022 at 6:05 PM To: Ce: Bloom PhD, Jesse D <{iii@fredhutch.org>, 487% <§@wh.iov.cn>, FS <Owh.iov.cn>, = Ney ecohealthalliance.org> Subject: Fw: RaTG13 sequence? Hi, Peter, I got the email below. I'm not able to read the article. What do you mean regarding the sequencing issue? Best regards, Zhengli, FRASER AE----- A4EX:"Bloom PhD, Jesse D" {i @fredhutch.org> AEIKAY [A] :2022-03-12 01:53:51 (EHAZN) We4E : wh. iov.cn" <ipwh.iov.cn>, '{§Jowh. iov.cn" (Owh iov.cn> Pik: +e: RaTG13 sequence? Hi Zhengli and Peng, I was just reading a new interview with Peter Daszak (https://theintercept.com/2022/03/1 1/covid-nih-ecohealthpeter-daszak-interview/). In it, he says there are parts of the RaTG13 genome that "aren't correctly sequenced." I was curious if you knew what he meant, in particular which parts are incorrect if the RaTG13 genome indeed isn't correctly sequenced as Peter is saying. I was especially wondering if the errors might be in the spike? We've been using the RBDs in experiments, and finding it doesn't bind any bat ACE2s we have tested particularly well (see Figure 1b of https://www.nature.com/articles/s41586-022-04464-z_reference.pdf). I was wondering if this could be affected by whatever sequencing problems Peter is referring to? Thanks, Jesse