Reading Room Production — page 90

September 2020: RACE for determination of the 5'end sequence" and "anal swab, not a fecal pellet, they used the High Pure Viral RNA kit for the RNA extraction" "for the 5'race done in September 2020 they used the viral RNA previously extracted from that anal swab sample." could go into the category called "description" for the Biosample accession of RaTG13 sample httos://www.ncbi.nim.nih.gov/biosample/?term=SAMN14082201, as in: https:/Awww.ncbi.nilm.nih.gov/biosample/SAMN02928182 2) The information about the "Method of genome sequence assembly" could go into the "Comment" section of the RaTG13 full sequence accession: https:/Awww.ncbi.nim.nih.gov/nuccore/1916859392 3) the list of primers used for amplicons and the points related to the amplicon names could go into the "Strategy" section of the SRA Experiment accession: https://www.ncbi.nlm.nih.gov/sra/SRX8357956 (see https://www.ncbi.nlm.nih.gov/sra/docs/submitmeta/ for details) From: Clare Thomas Sent:29 November 2021 09:37 To: wh.iov.cn Subject: RE: RE: Matters Arising regarding your Nature paper Dear Zhengli, Thank you very much. All the best, Clare From: g@wh.iov.cn <Biown iov.cn> Sent: 29 November 2021 09:07 To: Clare Thomas HB onature.com> Subject: Re: RE: Matters Arising regarding your Nature paper [External - Use Caution] Dear Clare, Please see our answers as follows: The reasons for the low bacterial reads in the raw sequencing data of RaTG13 could be: 1) The sample is anal swab, not a fecal pellet. 2) We used the High Pure Viral RNA Kit for the RNA extraction. 3) The sequencing depth was not high. For the 5'race done in September 2020, we used the viral RNA previously extracted from that anal swab sample. Best regards, Zhengli, Bown. iov.cn From: Clare Thomas Date; 2021-11-24 19:51 To: wh.iov.cn Subject: RE: Matters Arising regarding your Nature paper